Urea, N,N''-1,4-cyclohexanediylbis-, trans-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD Guideline and following the priciples of Good Laboratory Practice

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[(1r,4r)-4-(carbamoylamino)cyclohexyl]urea

EC Number:

813-604-1

Cas Number:

68533-01-7

Molecular formula:

C8 H16 N4 O2

IUPAC Name:

[(1r,4r)-4-(carbamoylamino)cyclohexyl]urea

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

batch number: 492-14-01.
the purity is 99.08 area % (190 nm) and 98.17 area % (200 nm)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organism, Daphnia magna Straus originated from the standard laboratory culture cultivated at the Institute of Industrial Organic Chemistry, Branch
Pszczyna, Department of Ecotoxicology[SOP/W/67]. Only organisms up to 24 hours old (not first brood progeny) and in good physiological
condition were used in the tests.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

19.1 – 20.4ºC

pH:

7.28 – 7.30

Dissolved oxygen:

9.00 – 9.01 mg/L (initial)
8.75 – 8.76 mg/L (final)

Nominal and measured concentrations:

120 mg/L, plus the control

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The LOEC/48 h is higher than the test item concentration of 120 mg/L.
The NOEC/48 h is higher than or equal to the test item concentration of 120 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes