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EC number: 813-180-8 | CAS number: 5856-32-6
The percent biodegradation in the toxicity control, containing both the test item and the reference item, was calculated based on the sum of the total organic carbon content (TOC) of the test item and the reference item.
In the toxicity control, the CO2 formation over the 19 day exposure period correlated very well with the CO2 evolution from the two added substances, i.e. test item and reference item. Within 14 days of exposure, biodegradation amounted to 78 %, reaching 83 % at the end of the test. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 20.8 mg/L because biodegradation in the toxicity control was ≥ 25 % within 14 days of incubation.
The tabulated values represent rounded results obtained by calculation using the exact raw data.
Table 1 Inorganic Carbon (IC Concentrations) Measured in the Absorber Flasks
Table 2 Absolute Amount of Inorganic Carbon (IC) Produced
Table 3 Biodegradation of the Test Item and the Reference Item
1: Based on values presented in Table 2, corrected for the inoculum controls
Table 4 pH of the Test Solutions or Suspensions
Figure 1: Biodegradation of the Test Item 10-HSA and the Reference Item Sodium benzoate during the Incubation Period
A: Overview over the whole test series
B: 10-day window for the biodegradation of the test item
(see picture below under section Illustration)
The test item 10-HSA was investigated for its ready biodegradability in a 28 Day CO2 Evolution (Modified Sturm) Test according to the OECD Guideline for Testing of Chemicals, No. 301 B (1992), the Method C.4-C of Commission Regulation (EC) No 440/2008 and the US EPA OPPTS 835.3110 Ready Biodegradability, Paragraph (m) (1998).
In accordance with the test guidelines the test was ended after 19 days since the biodegradation curves of the test item had reached a plateau over at least three determinations.
The test item 10-HSA was found to be biodegradable by 85 % under the test conditions within the 19 day exposure period.
The pass level for ready biodegradability, i.e. a CO2 formation of at least 60 % of the total organic carbon (TOC) in a 10 day window within the normally 28 day period of the test, was reached by exposure day 7 (62 %). In conclusion, 10-HSA was found to be readily biodegradable under the test conditions within 19 days.
In the toxicity control, containing both 10-HSA and the reference item sodium benzoate, biodegradation reached 78 % of the overall added TOC within 14 days of exposure. Thus, 10-HSA had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 20.8 mg/L.
In the procedure controls, average biodegradation of the reference item was 72 % by Exposure Day 5, and 90 % by Exposure Day 14, thus confirming suitability of the activated sludge (≥ 60 % degradation by Exposure Day 14). By the end of the test (Exposure Day 19), average biodegradation was 92 %. The validity criteria were fulfilled.
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