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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
specific investigations: biochemical or cellular interactions
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity
Author:
Sparnins VL, Lam LKT and Wattenberg LW
Year:
1984
Bibliographic source:
Biochem. Biophys. Res. Commun. 120, 2, 637-640

Materials and methods

Principles of method if other than guideline:
The effects of cyclic 12-, 8- and 6-carbon  compounds  (incl. 1,5-cyclooctadiene) on the glutathione S-transferase  (E.C 2.5.1.18) activity in the liver, intestinal mucosa and the  forestomach of female ICR/Ha mice were investigated.
GLP compliance:
no
Type of method:
in vivo
Endpoint addressed:
basic toxicokinetics

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
1,5-Cyclooctadiene from Aldrich Chemical Company, purity not reported

Test animals

Species:
mouse
Strain:
ICR
Sex:
female
Details on test animals and environmental conditions:
TEST ORGANISM
- Female ICR/Ha mice
- Age: 7 weeks
- Number: 5-10 animals per group
- Control: diet

Administration / exposure

Route of administration:
oral: feed
Vehicle:
unchanged (no vehicle)
Details on exposure:
TREATMENT - 30 or 50 µmol/g in diet for 2 weeks
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
every time
Post exposure period:
no
Doses / concentrations
Remarks:
Doses / Concentrations:
30 or 50 µmol/g diet
Basis:
nominal in diet
No. of animals per sex per dose:
5 - 10
Control animals:
yes, plain diet
Details on study design:
no further details

Examinations

Examinations:
EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small  bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as  substrate
Positive control:
no

Results and discussion

Details on results:
None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds 
were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated.

Any other information on results incl. tables

None of the compounds elicited increased GST activity in the forestomach.  

C6 ring compounds showed no significant effect. C12 ring compounds were  more 

effective than C8 ring compounds with a decrease in activity in the  order:
unsaturated > epoxide > alcohol > saturated.
-----------------------------------------------------------
Test compound          Liver              Small Bowel Mucosa
                    Specific Activity      Specific Activity
------------------------------------------------------------
None                1.96 +- 0.16           0.61 +- 0.04
1,5-Cyclooctadiene  2.75 +- 0.26*          0.76 +- 0.03*    
                                    * = p <0.005

Applicant's summary and conclusion

Conclusions:
1,5-cyclooctadiene was few effective in producing increased GST activity in the liver.
Executive summary:

Glutathione S-transferase is considered as a major detoxification system  which catalyzes conjugation of electrophilic compounds, e.g. chemical  carcinogens, to glutathione. The effects of cyclic 12-, 8- and 6-carbon  compounds  (incl. 1,5-cyclooctadiene) on the

glutathione S-transferase  (E.C 2.5.1.18) activity in the liver, intestinal mucosa and the  forestomach of female ICR/Ha mice were investigated.

None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed 

no significant effect. C12 ring compounds were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated.