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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997-12-17 to 1997-12-19
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report Date:
1997

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
1992
Deviations:
not specified
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Test substance: cis,cis-1,5-cyclooctadiene (Hüls AG)
Batch B803, produced 10 October 1997
Purity 99.6 % (GC-FID area)
Sample No. 1672/971103
Sample ID 0637/81865

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples for test substance analysis were taken at the start (0 hours) and at the end of the test (48 hours)

Test solutions

Vehicle:
no
Details on test solutions:
STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Concentration: 1 g/l stirred in deionized water for 18 hours and  filtered, no vehicle or solvent; analytical concentration 87.3 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISMS
- Strain: Daphnia magna Straus, clone 5
- Source/supplier: Hüls AG (inhouse)
- Breeding method: in 1 l beakers with M4 medium (Elendt, 1990), water renewal each 2-3 days, isolation of offspring for further breeding each ca. 
4 weeks
- Age: < 24 hours
- Feeding: Scenedesmus subspicatus, daily as much as consumed
- Pretreatment: Filtration of adults 24 h prior to testing - Feeding during test: no

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20 +/- 1 degree C
pH:
7.7 - 8.1
Dissolved oxygen:
7.5 - 8.4 mg/l
Nominal and measured concentrations:
0.79, 1.1,  1.7,  2.6,  3.9, 6.1, 8.7 mg/l (nominal)   
0.43, 0.72, 1.1,  1.8,  2.8, 4.8, 7.7 mg/l (analysis, 0 h)   
0.37, 0.51, 0.63, 0.95, 1.5, 2.6, 3.1 mg/l (analysis, 48 h)
Details on test conditions:
TEST SYSTEM
- Renewal of test solution: no
- Exposure vessel type: round-bottom test tubes with 10 ml test solution  and rubber plug to avoid volatilization
- Number of replicates, individuals per replicate: 4 replicates with 5 individuals each
- Control group: 2 reference substance controls (1.0 and 2.0 mg/l), one  blank
- Adjustment of pH: no
- Photoperiod: dark

DILUTION WATER
- Source: Synthetic: CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 6 H2O: 114 mg/l; NaHCO3: 65 mg/l; KCl: 6 mg/l
- Ca/Mg ratio: 4:1 - Na/K ratio: 10:1
- Aeration: no

TEST PARAMETER: immobilisation
- the mean of the analytical values at 0 and 48 hours was used for the evaluation.
Reference substance (positive control):
yes
Remarks:
potassium dichromate, CAS RN 7778-50-9

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.83 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.1 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Due to the steep dose response response curve the EC50-value was determined graphically.
EC0: highest concentration tested resulting in <10% effects
EC100: lowest concentration tested resulting in 100% effects
Results with reference substance (positive control):
- Concentrations: 1.0 mg/l / 2.0 mg/l
- Results: 35 % immobile / 100 % immobile (24 hours)

Any other information on results incl. tables

RESULTS: EXPOSED, CONTROL
- Effect data (Immobilisation): No. of immobile daphnia
  Control:    0 (24 h) /   0 (48 h)
  0.40 mg/l:  0 (24 h) /   0 (48 h) 
  0.61 mg/l:  0 (24 h) /   0 (48 h)
  0.83 mg/l:  0 (24 h) /   0 (48 h) 
  1.3  mg/l: 14 (24 h) /  15 (48 h) 
  2.0  mg/l: 20 (24 h) /  20 (48 h) 
  3.5  mg/l: 20 (24 h) /  20 (48 h) 
  4.9  mg/l: 20 (24 h) /  20 (48 h) 
  EC50 (24 hours) = EC50 (48 hours) = 1.1 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The EC50 (48 hours) was determined as 1.1 mg/L indicating that the test substance may be toxic to aquatic invertebrates.
Executive summary:

The acute toxicity of 1,5 -cyclooctadiene on Daphnia magna was investigated in a 48 -h test by Hüls AG (1998) according to EU method C.2. Seven concentrations ranging from 0.4 mg/L to 4.9 mg/L (mean values of measured concentrations) plus controls were tested. The following endpoint was derived:

EC50 (48 hours): 1.1 mg/L

The study was assessed as "reliable without restrictions".