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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1985-10-01 to 1985-10-03
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with national standard methods with acceptable restrictions (no solvent control, no analytical monitoring)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412, part 15
Principles of method if other than guideline:
Method: DIN 38412 part 15
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- cis,cis-1,5-cyclooctadiene of Hüls AG
- purity not reported

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Dispersion: MARLOWET EF (castor oil, ethoxylated, 40 EO) was added as solubilizer
- Concentration of vehicle/ solvent: 10 g test substance / l,  concentration of solubilizer not reported

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISMS
- Strain: Leuciscus idus melanotus HECKEL
- Supplier: Fischzucht Eggers, Hohenwestedt (Germany)
- Wild caught: no
- Age/size/weight/loading: length 6 cm +/- 2 cm
- Feeding: daily 3 % of body weight TetraMin
- Pretreatment: single treatment with Zephirol 1:50,000 for 1 hour  followed by 14 days under quarantine
- Feeding during test: no

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
- ca. 15 degree dH
Test temperature:
- Test temperature: 20 +/- 1 degree C
pH:
- pH: 7.3-7.7
Dissolved oxygen:
- Dissolved oxygen: 8.2-8.7 mg/l
Nominal and measured concentrations:
- Concentrations: 6, 8, 10, 13, 16 mg/l (nominal)
Details on test conditions:
DILUTION WATER
- Source: dechlorinated drinking water
- Aeration: continuous

TEST SYSTEM
- Renewal of test solution: daily
- Exposure vessel type: 10 l solution in 18 l aquarium
- Number of replicates, fish per replicate: 1, 10
- Photoperiod: 8 hours light / 16 hours dark
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
6 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
9.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 6.4-13.4 mg/L
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
16 mg/L
Details on results:
LC0 (48 h): 6 mg/L (highest concentration at which mortality was <10%)
LC100 (48 h): 16 mg/L (lowest concentration at which mortality was 100%)

Any other information on results incl. tables

- Effect data (Mortality): after 48 hours
   6 mg/l:   0 % dead
   8 mg/l:  50 % dead
  10 mg/l:  60 % dead
  13 mg/l:  80 % dead
  16 mg/l: 100 % dead
  95 % confidence interval of LC50: 6.4 - 13.8 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 (48 hours) was determined as 9.4 mg/L indicating that the test substance may be toxic to fish.
Executive summary:

The acute toxicity of 1,5 -cyclooctadiene on Leuciscus idus was tested in a semi-static 48h-test by Hüls AG (1995) according to DIN 38412, part 15. Five concentrations ranging from 6.0 to 16.0 mg/L were tested. The following endpoint was derived:

LC50 (48 hours):       9.4 mg/L (CL: 6.4 -13.4 mg/L)

The study was assessed as "reliable with restriction".