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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25-09-2017 to 29-09-2017
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical Benzene-1,4-diammonium sulphate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material (IUPAC name): Benzene-1,4-diammonium sulphate- Molecular formula: C6H8N2.H2O4S- Molecular weight: 206.221 g/mol- Smiles notation: S(=O)(=O)(O)O.c1(ccc(cc1)N)N- InChI: 1S/C6H8N2.H2O4S/c7-5-1-2-6(8)4-3-5;1-5(2,3)4/h1-4H,7-8H2;(H2,1,2,3,4)- Substance type: Organic - Physical state: Solid
Analytical monitoring:
not required
Remarks:
As substance is stable
Vehicle:
not specified
Details on test solutions:
The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L,1.25mg/L, 2.5mg/L,5mg/L respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio- Source: Unique Aqua Fish Corner- Length at study initiation (length definition, mean, range and SD): average 1.93 cm- Weight at study initiation (mean and range, SD): average 0.73 g -Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.-Duration of housing: 15 days -Photoperiod: 16 hours light and 8 hours dark-Diet : Standard brand feed, once daily-Acclimatization: seven days -feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
150 mg of CaCO3
Test temperature:
23 °C
pH:
7.2 - 7.6
Dissolved oxygen:
7.6 mg/l
Nominal and measured concentrations:
0.3125mg/L, 0.625mg/L, 1.25mg/L, 2.5mg/L and 5mg/L nominal concentration were used
Details on test conditions:
TEST SYSTEM - Test vessel: Plastic aquaria - Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water - Aeration: Aeration in test vessels was provided 1 day before the start of experiment - No. of organisms per vessel: 8 fishes OTHER TEST CONDITIONS - Photoperiod: 16 hours light and 8 hours dark
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 2.5 - < 5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 100 % mortality were observed at 5 mg/l
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: No effect were observed

Fig: 1. Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.1562

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.3125

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.625

4

4

-

-

-

-

-

-

-

-

-

-

-

-

1.25

4

4

-

-

-

-

-

-

-

-

-

-

-

-

2.5

4

4

-

-

-

-

-

-

-

-

4

4

4

4

Fig: Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

 

 

 

3hr

6hr

24hr

48hr

72hr

96hr

 

4

4

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.1562

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.3125

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.625

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

1.25

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

2.5

4

4

NS4

NS4

NS4

NS4

NS4

NS4

M4

M4

-

-

-

-

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

pH and oxygen content:

 

 

Number of

pH readings after

 

 

 

 

Nominal

fish

24h

 

48h

 

72h

 

96h

 

Concentration

 

 

V1

V2

V1

V2

V1

V2

V1

V2

(mg/l)

 

 

 

 

 

 

 

 

 

 

Control

4

4

7.7

7.7

7.8

7.8

8.0

8.0

8.1

8.1

0.3125

4

4

7.9

7.9

8.0

8.0

8.0

8.0

8.1

8.1

0.625

4

4

7.9

7.9

8.0

8.0

8.1

8.1

8.1

8.1

1.25

4

4

7.9

7.9

8.0

8.0

8.1

8.1

8.1

8.1

2.5

4

4

7.9

7.9

8.1

8.1

8.1

8.1

8.1

8.1

5

4

4

8.0

8.0

8.1

8.1

-

-

-

-

Nominal

Number of

Oxygen content (mg/L) after

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.9

6.9

6.8

6.8

6.7

6.7

6.6

6.6

0.3125

4

4

6.8

6.8

6.7

6.7

6.7

6.7

6.5

6.5

0.625

4

4

6.8

6.8

6.7

6.7

6.6

6.6

6.5

6.5

1.25

4

4

6.8

6.8

6.7

6.7

6.6

6.6

6.5

6.5

2.5

4

4

6.8

6.8

6.7

6.7

6.6

6.6

6.5

6.5

5

4

4

6.8

6.8

6.7

6.7

-

-

-

-

8.3. Test temperature

 

 

 

 

 

 

 

 

Nominal

Number of

Temperature (°C) after

 

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

22.9

22.9

23.1

23.1

22.9

22.9

23.5

23.5

0.3125

4

4

22.9

22.9

23.1

23.1

22.9

22.9

23.5

23.5

0.625

4

4

22.9

22.9

23.1

23.1

22.9

22.9

23.5

23.5

1.25

4

4

22.9

22.9

23.1

23.1

22.9

22.9

23.5

23.5

2.5

4

4

22.9

22.9

23.1

23.1

22.9

22.9

23.5

23.5

5

4

4

22.9 22.9

23.1

23.1

-

-

-

-

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical Benzene-1,4-diammonium sulphate on Zebra Fish Danio rerio was determine to be > 2.5 to < 5 mg/l.
Executive summary:

Study was conducted to access the effect of test chemical Benzene-1,4-diammonium sulphate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L,1.25mg/L, 2.5mg/L,5mg/L respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical Benzene-1,4-diammonium sulphate on Zebra Fish Danio rerio was determine to be > 2.5 to < 5 mg/l. Test consider to be valid as it fulfills all necessary criteria as per the standards. Based on the LC50 value, it can be consider that the chemical Benzene-1,4-diammonium sulphate was toxic and can be consider to be classified as aquatic chronic 2 category as per the CLP classification criteria.

Description of key information

Study was conducted to access the effect of test chemical Benzene-1,4-diammonium sulphate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L,1.25mg/L, 2.5mg/L,5mg/L respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical Benzene-1,4-diammonium sulphate on Zebra Fish Danio rerio was determine to be > 2.5 to < 5 mg/l. Test consider to be valid as it fulfills all necessary criteria as per the standards. Based on the LC50 value, it can be consider that the chemical Benzene-1,4-diammonium sulphate was toxic and can be consider to be classified as aquatic chronic 2 category as per the CLP classification criteria.

Key value for chemical safety assessment

Additional information

Study was conducted to access the effect of test chemical Benzene-1,4-diammonium sulphate on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring for achieving test concentrations of 0.3125mg/L, 0.625mg/L,1.25mg/L, 2.5mg/L,5mg/L respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test chemical Benzene-1,4-diammonium sulphate on Zebra Fish Danio rerio was determine to be > 2.5 to < 5 mg/l. Test consider to be valid as it fulfills all necessary criteria as per the standards. Based on the LC50 value, it can be consider that the chemical Benzene-1,4-diammonium sulphate was toxic and can be consider to be classified as aquatic chronic 2 category as per the CLP classification criteria.