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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Between 20 March 2000 and 19 July 2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
yes
Remarks:
Some test fish were of larger size than recommended in the test guideline for zebra fish. This deviation is not considered relevant to the result.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Amines, C12-14-alkyl, isooctyl phosphates
EC Number:
269-119-5
EC Name:
Amines, C12-14-alkyl, isooctyl phosphates
Cas Number:
68187-67-7
Molecular formula:
C19H42NPO4 - C29H63NPO4
IUPAC Name:
Amines, C12-14-tert-alkyl, isooctyl mono phosphates
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Daily analysis of test concnetrations 4mg/l and 8 mg/l and the control by TOC determination

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra Fish
- Source: Bio International B.V (Netherlands)
- Age at study initiation): 17 - 18 weeks
- Length at study initiation : 2.5 - 3.5 cm

Study design

Test type:
static
Water media type:
freshwater
Remarks:
Synthetic freshwater in accordance with ISO
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
13.9dH
Test temperature:
21 - 22°C
pH:
7.6 - 8.1
Dissolved oxygen:
7.9 - 8.8 mg/l
Nominal and measured concentrations:
Nominal cocentrations 0.5, 1, 2, 4 and 8 mg/l
Details on test conditions:
Static test with test vessels 300 x 135 x 200mm and 5l test medium. photoperiod of 16 hours light / 8 hours dark.

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
96 h
Dose descriptor:
LL0
Effect conc.:
ca. 1 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LL100
Effect conc.:
ca. 2 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)
Details on results:
Please see attached backgroung material for mortality data and results of test concentrations.

Visible effects on appearance and behaviour of fish
At 2mg/l - After 2 hours 10 animals showed sluggish swimming action. After 24 hours 3 animlaa showed sluggish and abnormal swimming action and 6 animals showed sluggish swimming action. After 48 hours 3 animals showed sluggish and abnormal swimming action.

At 4 mg/l - After 2 hours 10 animals showed sluggish swimming action

At 8 mg/l - 4 animals were motionless and showed uncontrolled swimming action when touched.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The acute toxicity of the test item was determined in a 96-hour static test. The 96hour LL0 was estimated to be 1mg/l and the 96hour LL100 was estimated to be 2mg/l.
Executive summary:

The acute toxicity of the test item was determined in a 96-hour static test. Due to the heterogenous composition of the test item, for the purposes of the test, the test medium was prepared as a Water Accommodated Fraction (WAF). The results were based on nominal loading rates.

96 hour LL0 was estimated to be 1mg/l

96 hour LL100 was estimated to be 2mg/l.