Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06 June 1992 - 14 August 1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to OECD test guidelines and a statement of GLP compliance is included in the study report.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Di-trimethylolpropane, Di-TMP and 3,3,7,7-tetrahydroximethyl-5-oxanone (reported as ditrimethylolpropane in this summary)
- Lot/batch No.: 90128
- Stability under test conditions: 1% water solution stable for one week at 4ºC
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Not applicable

Test solutions

Vehicle:
no
Details on test solutions:
A 10000 mg/L stock solution was used to prepare the test media.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Toxicon's culture which originates from NIVA, Oslo, Norway.


Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Post exposure observation period:
Not applicable

Test conditions

Hardness:
Not applicable
Test temperature:
Measured temperature at start = 23.0; temperature at end of test = 25.0ºC.
pH:
pH at start of test (recorded) = 8.1 - 8.2; pH at end of test = 8.9 - 9.5.
Dissolved oxygen:
Not applicable
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 0 (control group) and 1000 mg/L.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 mL glass E-Bottles - 100 mL fill volume (therefore 150 mL headspace)

- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates):5


GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH: Not required - pH in controls and test solutions were within expected range.
- Photoperiod: Continuous illumination
- Light intensity and quality: 100µEm-2s-1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Chlorophyll measurement: Samples analysed for chlorophyll a fluorescence with samples taken at 0, 24, 48, and 72 hours.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: In a preliminary test it was found that the growth inhibition at 500 and 1000 mg/L was very low.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
(Inhibition)
Details on results:
The growth rates (u) in the control and 1000 mg/L treatment were 1.10 and 0.96. The % inhibition in the 1000 mg/L treatment was 12.5%.
Results with reference substance (positive control):
Not applicable
Reported statistics and error estimates:
Graphical estimation of the EC50
Growth rate calculated as per the OECD guidelines

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A 72 hours algal growth inhibition limit study, test species Selenastrum capricornutum, was performed to determine the toxic effects of the test substance ditrimethylolpropane. The test was conducted according to OECD test guideline 201, and performed to GLP. The 72 hour EC50 value, based on growth rate, was observed to be >1000 mg/L (the highest nominal exposure concentration tested).
Executive summary:

A 72 hours algal growth inhibition limit study, test species Selenastrum capricornutum, was performed to determine the toxic effects of the test substance ditrimethylolpropane. The test was conducted according to OECD test guideline 201, and performed to GLP. The 72 hour EC50 value, based on growth rate, was observed to be >1000 mg/L (the highest nominal exposure concentration tested).