Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study (no analytics, no GLP)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412 L 9
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyrazole
EC Number:
206-017-1
EC Name:
Pyrazole
Cas Number:
288-13-1
Molecular formula:
C3H4N2
IUPAC Name:
1H-pyrazole

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
- Method: Concentration of the stock solution was 100 mg/L.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: Desmodesmus subspicatus (former name: Scenedesmus subspicatus)
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): Supplier: Sammlung für Algenkulturen der Universität Goettingen


Study design

Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
23.2 °C
pH:
7.85-8.20
Nominal and measured concentrations:
0, 2.5, 5.0, 10.0, 25.0, 50.0 and 100.0 mg/L (nominal concentrations)
Details on test conditions:
- Initial cells density: 12000 to 13000 cells/mL
- Control end cells density: yes
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412/9

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: in vivo chlorophyll-a-fluorescence at 685 nm wavelength (as measure for cell number) after 0, 24, 48, 72, and 96 hours
- pH values: after 0 and 96 hours
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
70.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 49.3 - 101.0 mg/L
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
41.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 30.6 - 56.1 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
53.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 43.5 - 66.9 mg/L
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
26.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 20.9 - 33.5 mg/L
Details on results:
- Exponential growth in the control: yes
- Any stimulation of growth found in any treatment: no
- EC50 values were determined with appropriate statistical methods (Tallarida et al. 1979)

Applicant's summary and conclusion