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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
The toxicity of chloroform as determined by single and repeated exposure of laboratory animals
Author:
Torkelson TR, Oyen F, Rowe VK
Year:
1976
Bibliographic source:
American Industrial Hygiene Association Journal 37(12): 697-705

Materials and methods

Principles of method if other than guideline:
Method after: Patch-test, semi-occlusive
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Chloroform
EC Number:
200-663-8
EC Name:
Chloroform
Cas Number:
67-66-3
Molecular formula:
CHCl3
IUPAC Name:
chloroform
Test material form:
liquid

Test animals

Species:
rabbit
Strain:
not specified
Details on test animals or test system and environmental conditions:
no data given

Test system

Type of coverage:
semiocclusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
no data given
Duration of treatment / exposure:
24 hours
Observation period:
two weeks after exposure
Number of animals:
3 rabbits
Details on study design:
A cotton pad soaked with chloroform was bandaged on the shaved belly of three rabbits during 24 hours. No cleaning of the skin after exposure was reported.

Results and discussion

In vivo

Results
Irritation parameter:
erythema score
Basis:
mean
Time point:
other: Not given in the study
Score:
> 2 - < 3
Reversibility:
not fully reversible within: 14 days
Irritant / corrosive response data:
Slight hyperaemia with moderate necrosis and a resulting eschar formation. Healing appeared to be delayed on this site.

Any other information on results incl. tables

The skin irritation effects were not evaluated following common methods such as the Draize's method and scores given above were estimated on the basis of the description of effects.

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information
Conclusions:
It can be concluded that chloroform is irritating to the rabbit's skin.
Executive summary:

A skin irritation test was carried out with chloroform applying a cotton pad soaked with chloroform to the shaved skin of three rabbits for 24 hours. The test did not apply principles of generally accepted test guidelines. The dermal application resulted in slight hyperaemia with moderate necrosis and a resulting eschar formation. Healing appeared to be delayed on this site. From these findings it can be concluded that chloroform is irritating to the rabbit skin.