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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Data is from peer-reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Acute oral toxicity and repellency of 933 chemicals to House and Deer mice.
Author:
E.W.Schafer, Jr. and W.A.Bowles,jr
Year:
1985
Bibliographic source:
Arch. Environ.Contam.Toxicol. 14, 111-129 (1985)

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as below
Principles of method if other than guideline:
Acute oral toxicity and repellency study of 3,5-diaminobenzoic acid in mice
GLP compliance:
not specified
Test type:
other: No data
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Specific details on test material used for the study:
- Name of test material (as cited in study report): 3,5 Diaminobenzoic acid
- Molecular formula (if other than submission substance): C7H8N2O2
- Molecular weight (if other than submission substance): 152.1522 g/mole
- Substance type: Organic
- Physical state: Solid

Test animals

Species:
mouse
Strain:
other: Deer
Sex:
not specified
Details on test animals and environmental conditions:
- Weight at study initiation: 20 gms
- Diet (e.g. ad libitum): wheat seed

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Water , corn oil, 1% carbopol
Details on oral exposure:
3,5 Diaminobenzoic acid was administrated by gavage using Water , corn oil, 1% carbopol as carriers.
Doses:
1250 mg/kg
No. of animals per sex per dose:
2 to 4 animals were used per geometrically spaced dosage.
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 3 days
Statistics:
estimated by method of thompson and weil

Results and discussion

Preliminary study:
No data
Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 1 250 mg/kg bw
Based on:
test mat.
Remarks on result:
other: No mortality observed
Mortality:
No mortality was observed in treated mice at 1250 mg/kg
Clinical signs:
No data
Body weight:
No data
Gross pathology:
No data
Other findings:
No data

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
LD50 was considered to be > 1250 mg/kg bw when Deer mice were treated with 3,5-diaminobenzoic acid orally by gavage.
Executive summary:

In a acute oral toxicity study, Deer mice were treated wtih 3,5-diaminobenzoic acid in the concentration of 1250 mg/kg bw orally by gavage using Water , corn oil, 1% carbopol as carriers and observed for 3 days. No 50 % mortality was observed in treated mice at 1250 mg/kg bw. Therefore,  LD50 was considered to be > 1250 mg/kg bw when Deer mice were treated with 3,5-diaminobenzoic acid orally by gavage.