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EC number: 208-621-0 | CAS number: 535-87-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Data is from peer reviewed journal
- Reference:
- Composition 1
- Qualifier:
- according to
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Study of Photobacterium phosphoreum at 5, 15 or 30 min for exposure to chemical as 50% reduction of the light emission of the test bacteria in a given time.
- GLP compliance:
- no
- Test material information:
- Composition 1
- Specific details on test material used for the study:
- - EC name:3,5 Diaminobenzoic acid
- Name of test material (as cited in study report): 3,5-diaminobenzoic acid
- Molecular formula (if other than submission substance): C7H5NO4
- Molecular weight (if other than submission substance): 167.1195 g/mol
- Smiles notation (if other than submission substance): c1(cc(ccc1)[N+](=O)[O-])C(=O)O
- InChI: 1S/C7H5NO4/c9-7(10)5-2-1-3-6(4-5)8(11)12/h1-4H,(H,9,10)
- Substance type: Organic
- Physical state: Solid
- Analytical purity: 98% - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Photobacterium phosphoreum
- Details on inoculum:
- Strain : NRRL-B-11177
- Test type:
- not specified
- Water media type:
- freshwater
- Total exposure duration:
- 30 min
- Remarks on exposure duration:
- Experiment conduct at 5 min,15 min and 30 min
- Hardness:
- No data
- Test temperature:
- 15 deg.C
- pH:
- >5 to <9
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- 31.8 mg/l
- Details on test conditions:
- No data
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 31.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: 50% reduction of the light emission of the test bacteria in a given time
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The effect concentration i.e EC50 value for toxicity to microorganism species Photobacterium phosphoreum (Strain : NRRL-B-11177) for test chemical 3, 5 diaminobenzoic (535-87-5) acid was observed to be 31.8 mg/l at 30 min.exposure period.
- Executive summary:
Toxicity study of test chemical 3,5 Diaminobenzoic acid (Cas no. 535-87-5) for the luminescent marine bacterium Photobacterium phosphoreum(Strain : NRRL-B-11177)was determine by MicrotoxTM test.
Study conducted for 5, 15 or 30 min exposure and this test was determined at a temperature of 15 deg.C. and pH range >5 to <9 was used. Study was to calculate the effective concentration EC50 value of photobacterium phosphorum 50% reduction of the light emission.
At 30 min. exposure period EC50 value for toxicity to microorganism of test chemical 3, 5 diaminobenzoic (Cas no.535-87-5) acid was observed to be 31.8 mg/l.
Reference
Description of key information
Toxicity study of test chemical 3,5 Diaminobenzoic acid (Cas no. 535-87-5) for the luminescent marine bacterium Photobacterium phosphoreum(Strain : NRRL-B-11177)was determine by MicrotoxTM test.
Study conducted for 5, 15 or 30 min exposure and this test was determined at a temperature of 15 deg.C. and pH range >5 to <9 was used. Study was to calculate the effective concentration EC50 value of photobacterium phosphorum 50% reduction of the light emission.
At 30 min. exposure period EC50 value for toxicity to microorganism of test chemical 3, 5 diaminobenzoic (Cas no.535-87-5) acid was observed to be 31.8 mg/l.
Key value for chemical safety assessment
- EC50 or LC50 for microorganisms:
- 31.8 mg/L
Additional information
Three studies including experimental studies from peer reviewed journal and predicted data from validated models for micro organism endpoint of test chemical 3,5-Diaminobenzoic acid (CAS no. 535 -87 -5) with read across chemical which is closest to target using log kow as the primary descriptor were reviewed as follows:
First study from the peer reviewed journal (Water Poll. Res. J. Canada; 1991 Volume 26, No. 3, 361-431) indicate toxicity study of test chemical 3,5 Diaminobenzoic acid (Cas no. 535-87-5) for the luminescent marine bacterium Photobacterium phosphoreum(Strain : NRRL-B-11177)was determine by MicrotoxTM test.Study conducted for 5, 15 or 30 min exposure and this test was determined at a temperature of 15 deg.C. and pH range >5 to <9 was used. Study was to calculate the effective concentration EC50 value of photobacterium phosphorum 50% reduction of the light emission. At 30 min. exposure period EC50 value for toxicity to microorganism of test chemical 3, 5 diaminobenzoic (Cas no.535-87-5) acid was observed to be 31.8 mg/l.
Above Experimental data for target was supported by predicted report using QSAR toolbox version 3.4. The prediction indicate the toxicity to micro organism of the substance 3,5 Diaminobenzoic acid (Cas no. 535-87-5) to Tetrahymena pyriformis based on the effects observed in a static freshwater system during a 48 hr exposure. The Inhibition growth concentration (IGC50) for the substance 3,5 Diaminobenzoic acid is estimated to be 450.74 mg/L.Based on this value, it can be concluded that the test chemical 3,5 Diaminobenzoic acid may have no concern to micro organism toxicity for acute exposure period.
Last experimental study from authorative database (ECOTOX database) for read across 3-aminophenol (Cas no. 591-27-5) indicate the effect concentration (EC20) for the substance 3-Aminophenol (CAS no. 591-27-5) was determine to be 70 mg/l on micro organism species Tetrahymena thermophila (Ciliate Protozoa) on the basis of effect on Adenosine triphosphatase activity in static fresh water.
Thus all above available experimental and other supported reports arrive at a judgement as the test substance 3,5-Diaminobenzoic acid (CAS no. 535 -87 -5) may have showed toxic effect to micro organism for acute exposure time
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