2,3,5-trimethylphenol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Short term toxicity test was performed in Daphnia magna to study the immobilization study of < 72 h old daphnids in test tubes which is filled with 8ml with test chemical and make up to 10ml for 24 hrs.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): 2,3,5Trimethylphenol
- Molecular formula : C9 H12 O
- Molecular weight: 136.193 g/mol
- Smiles notation: c1(c(cc(C)cc1O)C)C
- InChl: 1S/C9H12O/c1647(2)8(3)9(10)56/h45,10H,13H3
- Substance type: Organic
- Physical state: Solid
- Analytical purity: >95%

Vehicle:

yes

Details on test solutions:

- Controls: Yes, reference control taken
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): potassium dichromate

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: IRCHA Laboratory
- Age at study initiation (mean and range, SD): < 72 h old
- Feeding during test: No feeding during test.
- Food type: Chlorella vulgaris
- Amount: 1.0 x 106 cells/daphnid/h
- Frequency: 1.0 x 106 cells/daphnid/h

Test type:

static

Water media type:

freshwater

Total exposure duration:

24 h

Hardness:

7.5 ± 0.4mg 1-1 as Ca; 5.2 + 0.3mg 1 -1 as Mg

Test temperature:

22 ± 1°C (At breeding room)

pH:

7

Details on test conditions:

- Test vessel: Test tubes
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume:10 ml
- Aeration: No aeration
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): duplicate with a
minimum of three replicates
- Photoperiod: 16 h daylight/8 h darkness (at breeding process)
- Range finding study: Yes
- Test concentrations: 0.1, 0.35, 1, 3.5, 10, 35, 100, and 350 mg l- 1

Duration:

24 h

Dose descriptor:

IC50

Effect conc.:

26.69 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0.155-0.236 mmol/l confidence limit

Validity criteria fulfilled:

not specified

Conclusions:

The IC 50 value for short term toxicity to aquatic invertebrates for 2,3,5 trimethylphenol (687-82-5) was determined to be 26.69 mg/l.

Executive summary:

Short term toxicity test was performed in Daphnia magna to study the immobilization study of < 72 h old daphnids in test tubes which is filled with 8ml with test chemical and make up to 10ml for 24 hrs. Range finding test was conducted and a concentration was decided with specific test conditions. From experiment it was concluded that the IC 50 value for short term toxicity to aquatic invertebrates for 2,3,5Trimethylphenol (687-82-5) was determined to be 26.69 mg/l. Based on the value, the chemical was considered to be toxic in nature but as it was readily biodegradable in nature , thus the 2,3,5Trimethylphenol was considered to be not classified as per the CLP regulations.

Description of key information

Short term toxicity test was performed in Daphnia magna to study the immobilization study of < 72 h old daphnids in test tubes which is filled with 8ml with test chemical and make up to 10ml for 24 hrs. Range finding test was conducted and a concentration was decided with specific test conditions. From experiment it was concluded that the IC 50 value for short term toxicity to aquatic invertebrates for 2,3,5Trimethylphenol (687-82-5) was determined to be 26.69 mg/l. Based on the value, the chemical was considered to be toxic in nature but as it was readily biodegradable in nature , thus the 2,3,5Trimethylphenol was considered to be not classified as per the CLP regulations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

26.69 mg/L

Additional information

Short term toxicity to aquatic invertebrates for 2,3,5Trimethylphenol (CAS:687-82-5)was summaries with one experimental study and two experimental studies with one read across substances 2,3-dimethylphenol are as follows:

Short term toxicity test was performed in Daphnia magna byJames devillers, 1988 to study the immobilization study of < 72 h old daphnids in test tubes which is filled with 8ml with test chemical and make up to 10ml for 24 hrs. Range finding test was conducted and a concentration was decided with specific test conditions. From experiment it was concluded that the IC 50 value for short term toxicity to aquatic invertebrates for 2,3,5Trimethylphenol (687-82-5) was determined to be 26.69 mg/l. Based on the value, the chemical was considered to be toxic in nature but as it was readily biodegradable in nature , thus the 2,3,5Trimethylphenol was considered to be not classified as per the CLP regulations.

From the same reference, study was performed on read across 2,3 –dimethylphenol in Daphnia magna when they were exposed for 24h.They were found to be immobilized at the concentration of 13.615mg/l .Therefore the IC50 was considered to be 13.615mg/l for 2,3 –dimethylphenol when Daphnia magna  were exposed for 24h under static condition.

For same read across 2,3 –dimethylphenol study was conducted by A. Kahru, 2000 to find out the toxicity of 2, 3-dimethyl phenol to Daphnia magna. Test plates with Daphnia magna neonates (Daphtoxkit Fᵀᴹ manga) were incubated for 48 h at 20 deg C in the dark. Immobilization of the organism was observed after exposure to 2, 3-dimethyl phenol. The LC50 value of 2,3-dimethylphenol was considered to be 11 mg/l for Daphnia magna. Based on the value, the chemical was considered to be toxic in nature but as it was readily biodegradable in nature , thus the 2,3,dimethylphenol was considered to be not classified as per the CLP regulations.

Based on the above experimental study for target and studies of read across chemicals it can be considered that all the above studies supported the target classification, so based on the results it was considered that 2,3,5Trimethylphenol (687-82-5) was not hazardous to aquatic invertebrates and can be considered to be not classified as per the CLP regulations.