Reaction product of diazotised 4-amino-5-hydroxy-2,7- naphthalenedisulfonic acid coupled with resorcinol, subsequently coupled with diazotised 2-amino-4,6-dinitrophenol and 4-nitrobenzenamine, chelated with iron (3+), sodium salts

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From July 17, 2000 to March 16, 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Qualifier:

according to guideline

Guideline:

other: Royal Decree 363/1995 (BOE no. 133 of June 1995)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Selenastrum capricornutum
- Strain: CCAP 278/4
- Source: Culture Collection Algae and Protozoo.
- Temperature: 22 ± 2 ºC
- Intensity and quality of illumination: 2500 Lux, the surface is made for receiving continuous and uniform fluorescent lighting/white cold type.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

22 ± 2 °C

pH:

7.15

Nominal and measured concentrations:

NOMINAL: 0.1 mg/l, 0.32 mg/l, 1 mg/l, 3.2 mg/l, 10 mg/l, 32 mg/l and 90 mg/l
MEASURED: 0.1 mg/l, 0.32 mg/l, 1 mg/l, 3.21 mg/l, 10 mg/l, 32.1 mg/l and 90.3 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 40 ml test tube
- Type (delete if not applicable): open
- Material, size: 10 ml
- Aeration: no
- Initial cells density: 1.18 x 10^4 cells/ml
- No. of vessels per concentration (replicates): 2
- No. of vessels per control: 1

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: test medium was prepared according (AFNOR T90-304) regulation.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

ca. 3.4 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Results with reference substance (positive control):

CI 50 72h = 1,05 mg/

The results of cell concentration are shown in the following table:

	Control		Test concentrations (mg/l)
Test	0 h	72 h	0.1	0.32	1	3.2	10	32	90
1	1.30 x 10^4	2.15 x 10^5	2.21 x 10^5	2.11 x 10^5	1.66 x 10^5	1.21 x 10^5	6.55 x 10^4	2.55 x 10^4	-
2	1.05 x 10^4	2.18 x 10^5	2.23 x 10^5	2.06 x 10^5	1.61 x 10^5	1.13 x 10^5	7.30 x 10^4	1.30 x 10^4	-
mean	1.18 x 10^4	2.14 x 10^5	2.22 x 10^5	2.08 x 10^5	1.63 x 10^5	1.17 x 10^5	6.93 x 10^4	1.93x 10^4	-
sd	1768	5303	1768	3536	3536	5303	5303	8839	-

Growth inhibition:

Concentration	% of inhibition
control	0
0.1	0
0.32	3.1
1	25.3
3.2	48.1
10	71.6
32	96.3
90	-

Conclusions:

The substance was tested for toxicity to aquatic algae following OECD 201. Under the experimental conditions the IC50(72h) = 3.4 mg/l of active material.

Executive summary:

The substance has been tested for acute toxicity on algae according to the OECD guideline 201 (1984) and Royal Decree 363/1995 (BOE no. 133 of June 1995).

For determination of cell growth inhibition freshwater of Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) has been used. The alga has been subjected to the substance at the test concentrations of 0.1mg/l, 0.32 mg/l, 1 mg/l, 3.2 mg/l,10 mg /l, 32 mg/l and 90 mg/l of active ingredient. The total test duration was 72 hours.

The result obtained in this test indicates that the dye had an IC 50 value (72h) equal to 3.4 mg/l of active material (nominal).

Conclusion

IC50(72h) = 3.4 mg/l

Description of key information

IC50 (72 h) = 3.4 mg/l of active material

Key value for chemical safety assessment

EC50 for freshwater algae:

3.4 mg/L

Additional information

OECD 201