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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: A reliable and scientifically sound guideline study referring to a read-across substance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Molecular weight: 136 g/mol
- Water solubility: 625 g/L at 25°C
- Appearance: white powder

Based on the molecular weights, a concentration of 1200 mg/L sodium trifluoroacetate/L corresponds to 1000 mg trifluoroacetate anion/L.

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0 and 1200 mg/L (nominal) in ISO water
- Sampling: 0h and 96h after test initiation
- Sample storage conditions before analysis: the samples were stored at cool and dark before analysis

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebra fish
- Source: Ruinemans, Montfoort, The Netherlands (commercial supplier)
- Length at study initiation (mean): 2.3 - 3.4 cm
- Weight at study initiation (mean): 0.23 g
- Feeding during test: no


ACCLIMATION
- Conditions: the zebra fishes were held in plastic containers of 40 x 60 X 40 cm containing ca. 80 L reconstitued water (ISO), continuously filtered
- Type of food: trout feed and water fleas

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Test temperature:
21.0 - 22.8°C
pH:
7.6 - 7.9
Dissolved oxygen:
8.3 - 8.7 mg/L
Nominal and measured concentrations:
Nominal: 1200 mg/L and control
Measured (mean): 1210 mg/L (at test start) and 1205 mg/L (at test end)
Details on test conditions:
TEST SYSTEM
- Test vessel: aquaria
- Fill volume: 4L ISO water
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: reconstitued water (ISO)
Deionised water (LRS 1322 830 78115), 294 mg/L CaCl2*2H2O, 123 mg/L MgSO4*7H2O, 64 mg/L NaHCO3, 5.8 mg/L KCl
(pH = 7.8; hardness (CaCO3 = ca. 250 mg/L))
- Intervals of water quality measurement: at the beginning (0h) and at the end of the study (96h)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h/light; 8h/dark

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 100, 300, 1000 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
Results with reference substance valid? yes
- EC50 (96h): 142 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes