4-methoxyphenylacetic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result performed using standard methods of analysis

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

This study was designed to assess the toxic effects of the test compound on the ZEBRA FISH (Danio rerio) .Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes each. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

GLP compliance:

no

Specific details on test material used for the study:

- IUPAC name: (4-methoxyphenyl)acetic acid
- Name of the test material: 4-methoxyphenylacetic acid
- Molecular formula: C9H10O3
- Molecular weight: 166.175 g/mol
- SMILES: c1(ccc(OC)cc1)CC(O)=O
- InChI: 1S/C9H10O3/c1-12-8-4-2-7(3-5-8)6-9(10)11/h2-5H,6H2,1H3,(H,10,11)
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 450 mg of the test substance in 4.5 liters of potable water (passed through reverse osmosis system) with 1hr stirring for achieving test concentrations of 100mg/L,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:ZEBRA FISH
- Source:UNIQUE AQUARIUM, NAGPUR
- Length at study initiation (length definition, mean, range and SD):average 1.63 cm
- Weight at study initiation (mean and range, SD):0.1899g (average)

ACCLIMATION
- Acclimation period:7 days
- Acclimation conditions (same as test or not):same as test
- Type and amount of food during acclimation:Standard brand feed
- Feeding frequency during acclimation:Daily once
- Health during acclimation (any mortality observed):No mortality

FEEDING DURING TEST
- Food type:Standard brand feed
- Frequency:once daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

148.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.05

Dissolved oxygen:

8.0 mg/l

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:The test vessels used were all plastic aquaria having the water capacity of 5liters.
- Material, size, headspace, fill volume: 2 liters
- Aeration: No
- No. of organisms per vessel:8

OTHER TEST CONDITIONS
- Adjustment of pH:No
- Photoperiod:16 hours light and 8 hours dark

TEST CONCENTRATIONS
- Test concentrations: 0,100 mg/l

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

A 96 h study was conducted for CAS No. 104-01-8 on Zebra fish(Danio rerio), at a test concentrations of 100mg/L . The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria.
The lethal concentrations LC50 was found to be >100 mg/L
LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L
LC50 (96 hours) Experimental = >100 mg/L

Sublethal observations / clinical signs:

Mortality and symptoms

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24h		48h		72		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Nominal Concentration (mg/l)

Number of fish

Mortality (cumulated) after

3 h

6 h

24h

48h

72

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.104-01-8 on ZEBRA FISH(Danio rerio)was found to be >100 mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 4-methoxyphenylacetic acid. The nominal concentration selected for the experiment were 100mg/L and test fish were exposed to these concentration for 96 hours. The median lethal concentration (LC50) for 4-methoxyphenylacetic acid on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >100 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, 4-methoxyphenylacetic acid (CAS No. 104-01-8) does not exhibit short term toxicity to fish.

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

Description of key information

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for 4-methoxyphenylacetic acid. The nominal concentration selected for the experiment were 100mg/L and test fish were exposed to these concentration for 96 hours. The median lethal concentration (LC50) for 4-methoxyphenylacetic acid on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >100 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, 4-methoxyphenylacetic acid (CAS No. 104-01-8) does not exhibit short term toxicity to fish.

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Various studies including experimental data following OECD guideline and predicted results from validated model for toxicity to fish with the target substance 4-methoxyphenylacetic acid (CAS no. 104-01-8) and to its relevant read across substance (4-chloro-2-methylphenoxy)acetic acid (CAS No. 94-74-6) by considering its structure-activity relationships, were summarized as follows:

 

Fish Acute Toxicity test according to OECD Guideline 203 was conducted by UERL (2017) for 4-methoxyphenylacetic acid. The nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours. The median lethal concentration (LC50) for 4-methoxyphenylacetic acid on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >100 mg/L.

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

 

Also, based on the Ecological Structure Activity Relationships (ECOSAR) Predictive Model version 1.11 (2017), the median lethal concentration (LC50) for the test substance, 4-methoxyphenylacetic acid, in fish was estimated to be 3765.923 mg/L on the basis of mortality effect in a 96 hour study.

Moreover, in another study for the read across substance (4-chloro-2-methylphenoxy)acetic acid (CAS No. 94-74-6) published in an authoritative database such as ECOTOX database (2017), the study was designed to assess the toxic effects of the test compound (4-chloro-2-methylphenoxy)acetic acid on the Salmo trutta in a 96 hrs. of exposure.In the experiment static condition maintained with 11 mg/L CaCO3 hardness, 10 Deg.C test temperature, 6.3 pH and 3.2 mS/m conductivity. On the basis of mortality effect the lethal concentration (LC50) on fish Salmo trutta (Brown Trout) was observed to be 300 mg/l. 

 

So, based on the above predicted and experimental studies for target substance 4-methoxyphenylacetic acid (CAS no. 104-01-8) and the read across substance, the lethal concentration LC50 was found to be in the range of >100 mg/L to 3765.923 mg/L. Thus, on the basis of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, 4-methoxyphenylac etic acid (CAS No. 104-01-8) does not exhibit short term toxicity to fish.