Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

biodegradation in water: ready biodegradability
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
See section 13
according to guideline
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
GLP compliance:
Oxygen conditions:
Inoculum or test system:
sewage, domestic, non-adapted
Details on inoculum:
Sludge of a domestic sewage treatment plant (ARA-Rhein) collected on 14 August 1990Sludge concentration: 1000 mg/l
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Water / Nutrient: according to the specification of the EEC L133 (pp. 99-105) Volume 31 resp. OECD Method 302B.Temperature: 22.0 ± 3°C Lighting: indirect daylightTwo tests are running in parallel
Reference substance:
diethylene glycol
Key result
other: bioelimination
Sampling time:
28 d
Remarks on result:
other: Adsorption after 3 hours: 33.4 %
Details on results:
The result is an average of two tests running in parallel. Adsorption after 3 hours: 33.4 %.
Results with refernce substance: 99.6 % after 12 days
Validity criteria fulfilled:
Interpretation of results:
not readily biodegradable
Bioelimination after 28 days was 33.5 % where the adsorption after 3 hours was 33.4 %.
The substance is considered to be non readily biodegradable.
Executive summary:

Biodegradation test was carried out according to OECD guideline 302B. The bioelimination value was 33.5 % (average of two tests running in parallel) after 28 days, where the adsorption after 3 hours was 33.4 %.

Description of key information

Not readily biodegradable

Key value for chemical safety assessment

Additional information

The probability of rapid biodegradation of the test substance has been calculated by QSAR Dyes R&C prediction tools. The overall assessment is based on the responses given by different predictive models for biodegradability under various conditions. The substance was predicted to be not ready biodegradable.

Moreover, being the substance a dye, it is not intended to be readily biodegradable, by the nature of its design and use.