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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Justification for type of information:
see section 13
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across: supporting information
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic, non-adapted
Details on inoculum:
Sludge of a domestic sewage treatment plant (ARA-Rhein) collected on 14 August 1990 Sludge concentration: 1000 mg/l
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Water / Nutrient: according to the specification of the EEC L133 (pp. 99-105) Volume 31 resp. OECD Method 302B.Temperature: 22.0 ± 3°C Lighting: indirect daylightTwo tests are running in parallel
Reference substance:
diethylene glycol
Key result
Parameter:
other: bioelimination
Value:
33.5
Sampling time:
28 d
Remarks on result:
other: Adsorption after 3 hours: 33.4 %
Details on results:
The result is an average of two tests running in parallel. Adsorption after 3 hours: 33.4 %
Results of reference substance: 99.6 % after 12 days
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Bioelimination after 28 days was 33.5 %, adsorption after 3 hours was 33.4 %.
The substance is considered as non readily biodegradable
Executive summary:

Biodegradation test was carried out according to OECD guideline 302B. The bioelimination value was 33.5 % (average of two tests running in parallel) after 28 days, where the adsorption after 3 hours was 33.4 %.

Description of key information

Not readily biodegradable

Key value for chemical safety assessment

Additional information