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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
secondary literature
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: Refer below principle
Principles of method if other than guideline:
The antibacterial activities of twenty-one oxygenated monoterpenes including test chemical menthone was determine against 63 bacterial strains by disc diffusion method.
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of the test material: 2-isopropyl-5-methylcyclohexanone
- Molecular formula: C10H18O
- Molecular weight: 154.2512 g/mol
- Substance type: Organic
- Physical state: Liquid
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):methanol
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):1 ml o
Test organisms (species):
other: Clavibacter michiganense,Xanthomonas campestrispv. rhapontici,Klebsiella trevisanii,Pseudomonas aeruginosa ATCC 9027
Details on inoculum:
- Laboratory culture:Bacterial cultures were preserved in Luria Broth and 15% glycerol solution at -80 deg.C prior to use.
- source of Microorganisms:Microorganisms were provided from the Department of Clinical Microbiology, Faculty of Medicine and Plant Diagnostic Laboratory,Faculty of Agriculture, Ataturk University,Erzurum, Turkey.
- Method of cultivation:in petri dish
- Preparation of inoculum for exposure:Suspensions (100 μl) of the bacteria, adjusted to 108 cfu/ml final cell concentration, were added to flasks containing 25 ml sterile NA medium and then poured into Petri dishes and spread by a sterile swab (9 cm). 30 mg of each of the compounds were dissolved in 1 ml of methanol and these solutions were sterilized in 0.45 μm milipore filters. Sterilized discs (5 mm) were soaked with 10 μl of each compound solution. These discs were put in the middle of plates containing NA medium.
Penicillin was used as a positive control. For this purpose, 1 mg of penicillin was added into 1 ml sterilized water, and a sterilized disc was soaked with 10 μl of this solution. Bacterial cultures of plant origins were incubated at (27 deg.C), whereas the bacterial cultures of clinic and food origins were incubated at (35 Deg.C) for 6 d. At the end of six-day-periods, inhibition zones were measured in mm. All the tests were made in triplicate.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
6 d
Hardness:
No data
Test temperature:
27 deg.C
Dissolved oxygen:
No data
Salinity:
No data
Conductivity:
No data
Nominal and measured concentrations:
10 μl
Details on test conditions:
Suspensions (100 μl) of the bacteria, adjusted to 108 cfu/ml final cell concentration, were added to flasks containing 25 ml sterile NA medium and then poured into Petri dishes and spread by a sterile swab (9 cm). 30 mg of each of the compounds were dissolved in 1 ml of methanol and these solutions were sterilized in 0.45 μm milipore
filters. Sterilized discs (5 mm) were soaked with 10 μl of each compound solution. These discs were put in the middle of plates containing NA medium.
Penicillin was used as a positive control.
For this purpose, 1 mg of penicillin was added into 1 ml sterilized water, and a sterilized disc was soaked with 10 μl of this solution. Bacterial cultures of plant origins were incubated at (27 deg.C), whereas the bacterial cultures of clinic and food origins were incubated at (35 deg.C) for 6 d. At the end of six-day-periods,inhibition zones were measured in mm. All the tests were made in triplicate.
Reference substance (positive control):
yes
Remarks:
penicillin
Key result
Duration:
6 d
Dose descriptor:
other: MIC
Effect conc.:
10 other: μl
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Remarks on result:
other: showed effects on 4 bacterial strain out of 63 strain tested.

Bacterial species

Antibacterial activities of Menthone as diameter of average inhibition zone (mm)

Clavibacter michiganense

9

Xanthomonas campestrispv.rhapontici

7

Klebsiella trevisanii

8

Pseudomonas aeruginosa ATCC 9027

7

Validity criteria fulfilled:
not specified
Conclusions:
In experiment the test substance Menthone (Cas no.1074-95-9) showed inhibitory effects (MIC) on growth of the 4 bacterial strains (Clavibacter michiganense, Xanthomonas campestrispv.Rhapontici,Klebsiella trevisanii,Pseudomonas aeruginosa ATCC 9027)out of 63 strains at a concenttration of 10μl.
Executive summary:

The objective of this study was to evaluate the inhibitory effects of 21 pure oxygenated monoterpenes, obtained commercially including test chemical Menthone(CAS No.1074-95-9) on the growth of 63 bacterial strains (plant, food and clinic origins).

 

In experiment Suspensions (100μl) of the bacteria, adjusted to 108 cfu/ml final cell concentration, were added to flasks containing 25 ml sterile NA medium and then poured into Petri dishes and spread by a sterile swab (9 cm). 30 mg of each of the compounds were dissolved in 1 ml of methanol and these solutions were sterilized in 0.45μm milipore filters. Sterilized discs (5 mm) were soaked with 10μl of each compound solution. These discs were put in the middle of plates containing NA medium.Penicillin was used as a positive control.For this purpose, 1 mg of penicillin was added into 1 ml sterilized water, and a sterilized disc was soaked with 10μl of this solution. Bacterial cultures of plant origins were incubated at (27 ± 2) °C, whereas the bacterial cultures of clinic and food origins were incubated at (35 ± 2) °C for 6 d. At the end of six-day-periods, inhibition zones were measured in mm. All the tests were made in triplicate.

 

At end of the experiment the test substance Menthone showed inhibitory effects on growth of the 4 bacterial strains (Clavibacter michiganense, Xanthomonas campestrispv.Rhapontici, Klebsiella trevisanii, Pseudomonas aeruginosa ATCC 9027)out of 63 strains at a concenttration of 10μl.

Description of key information

In experiment the test substance Menthone (Cas no.1074-95-9) showed inhibitory effects (MIC) on growth of the 4 bacterial strains (Clavibacter michiganense, Xanthomonas campestrispv.Rhapontici,Klebsiella trevisanii,Pseudomonas aeruginosa ATCC 9027)out of 63 strains at a concenttration of 10μl.

Key value for chemical safety assessment

Additional information

The objective of this study was to evaluate the inhibitory effects of 21 pure oxygenated monoterpenes, obtained commercially including test chemicalMenthone(Cas no.1074-95-9) on the growth of 63 bacterial strains (plant, food and clinic origins).

 

In experiment Suspensions (100μl) of the bacteria, adjusted to 108 cfu/ml final cell concentration, were added to flasks containing 25 ml sterile NA medium and then poured into Petri dishes and spread by a sterile swab (9 cm). 30 mg of each of the compounds were dissolved in 1 ml of methanol and these solutions were sterilized in 0.45μm milipore filters. Sterilized discs (5 mm) were soaked with 10μl of each compound solution. These discs were put in the middle of plates containing NA medium.Penicillin was used as a positive control.For this purpose, 1 mg of penicillin was added

into 1 ml sterilized water, and a sterilized disc was soaked with 10μl of this solution. Bacterial cultures of plant origins were incubated at (27 ± 2) °C, whereas the bacterial cultures of clinic and food origins were incubated at (35 ± 2) °C for 6 d. At the end of six-day-periods, inhibition zones were measured in mm. All the tests were made in triplicate.

 

At end of the experiment the test substanceMenthone showed inhibitory effects on growth of the 4 bacterial strains (Clavibacter michiganense, Xanthomonas campestrispv.Rhapontici,Klebsiella trevisanii,Pseudomonas aeruginosa ATCC 9027)out of 63 strains at a concenttration of 10μl.