5-amino-2-anilinobenzenesulphonic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Mutagenicity testing of some commonly used dyes.

Author:

K T Chung, G E Fulk and A W Andrews

Year:

1981

Bibliographic source:

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Oct. 1981, p. 641-648

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: refer below guideline

Principles of method if other than guideline:

Gene mutation toxicity study was performed for sodium 4-aminonaphthalene-1-sulphonate

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 4-aminonaphthalene-1-sulphonate

EC Number:

204-975-5

EC Name:

Sodium 4-aminonaphthalene-1-sulphonate

Cas Number:

130-13-2

IUPAC Name:

sodium 4-aminonaphthalene-1-sulfonate

Details on test material:

- Name of the chemical: sodium 4-aminonaphthalene-1-sulphonate
- Molecular formula: C10H9NO3S.Na
- Molecular weight: 245.233 g/mol
- Substance type: Organic
- Purity: No data available

Method

Target gene:

Histidine gene

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA1535, TA1537, TA1538, TA98, and TA100

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

Liver homogenates (S9) were prepared from male Sprague-Dawley rats stimulated with Aroclor 1254

Test concentrations with justification for top dose:

0, 5 - 5000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: Dimethylsulphoxide
- Justification for choice of solvent/vehicle: No data

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

sodium azide

other: 2-Aminoanthracene

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

DURATION
- Preincubation period: No data
- Exposure duration: No data
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data

SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data

NUMBER OF REPLICATIONS: Duplicate

NUMBER OF CELLS EVALUATED: No data

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data

OTHER EXAMINATIONS:
- Determination of polyploidy:
- Determination of endoreplication:
- Other:

OTHER:

Evaluation criteria:

A compound was considered mutagenic when the number of revertants above background was at least twice the value of the historical control mean or twice the value of the current control mean, whichever was greater

Statistics:

No data

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA1535, TA1537, TA1538, TA98, and TA100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

No data available

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with and without

Sodium 4-aminonaphthalene-1-sulphonate failed to induce mutation in the Salmonella typhimurium tester strains TA1535, TA1537, TA1538, TA98,or TA100 with and without male rat Aroclor S9 mix and hence is not likely to classify as a gene mutant in vitro.

Executive summary:

Gene mutation toxicity study was performed for sodium 4-aminonaphthalene-1-sulphonate using the Salmonella typhimurium tester strains TA1535, TA1537, TA1538, TA98 and TA100 with and without male rat Aroclor S9 mix in the plate incorporation assay. The read across chemical was dosed at dose value from 5 - 5000 µg/plate. Concurrent vehicle control and appropriate positive controls were also incorporated on the study. Sodium 4-aminonaphthalene-1-sulphonate failed to induce mutation in the Salmonella typhimurium tester strains TA1535, TA1537, TA1538, TA98,or TA100 with and without male rat Aroclor S9 mix and hence is not likely to classify as a gene mutant in vitro.