5-amino-2-anilinobenzenesulphonic acid

Endpoint summary

Additional information

Short-term toxicity to fish:

Predicted model data and experimental study results of short term fish for the target chemical 5-amino-2-anilinobenzenesulfonic acid (CAS No. 91-30-5) with respective read across were reviewed to summarize the following information:

In key study the 96 hrs aquatic toxicity study was predicted to assess toxic effects of the test compound 5-amino-2-anilinobenzenesulfonic acid (CAS no. 91-30-5) and the result were predicted (SSS QSAR Prediction model, 2016). The study was based on the effects of the test compound on Danio rerio fish in a static fresh water system. The predicted data suggests the lethal concentration (LC50) for the test compound was estimated to be 195.985412598 mg/l on the basis of mortality effect.       

 

Above predicted lethal concentrations supported by experimental study (Sustainability Support Services (Europe) AB- UERL laboratory; 2016) for read across chemical disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate (Cas no. 3567-66-6) which is indicates Fish Acute Toxicity test according to OECD Guideline 203 was conducted for chemical disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate on fish species Danio rerio in static fresh water condition. The nominal concentration of selected for the experiment were 100 mg/l and Zebra fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L.

Authorative database Data bank of Environmental Properties of Chemicals (EnviChem), 2016 for various read across substance showed following results:

For read across Sodium 4-aminonaphthalene-1-sulphonate (Cas no130-13-2) indicate the Fish toxicity study was conducted for 48 hrs for evaluating the lethal concentration of test substance Sodium 4-aminonaphthalene-1-sulphonate on fish Oryzias latipes on the basis of mortality effect.During experiment the lethal concentration (LC50) for test substance Sodium 4-aminonaphthalene-1-sulphonate was found to be >4000 mg/l.

Read across chemical 7-Amino-4 hydroxy- 2 – naphthalenesulphonic acid (Cas no. 87-02-5) indicate that the Fish toxicity study was conducted for 48 hrs for evaluating the lethal concentration of test substance 7-Amino-4 hydroxy- 2 – naphthalenesulphonic acid on fish Oryzias latipes on the basis of mortality effect.During experiment the lethal concentration (LC50) for test substance 7-Amino-4 hydroxy- 2 – naphthalenesulphonic acid was found to be 480 mg/l.

And read across substance 2-Amino-8-naphthol-6-sulfonic acid (Cas no.90-51-7) indicate Fish toxicity study was conducted for 48 hrs for evaluating the lethal concentration of test substance 2-Amino-8-naphthol6-sulfonic acid on fish Oryzias latipes on the basis of mortality effect.During experiment the lethal concentration (LC50) for test substance 2-Amino-8-naphthol6-sulfonic acid was found to be 335 mg/l.

The above experimental results, are further supported by one more read across 6-aminonaphthalene-1,3-disulphonic acid (Cas no. 118-33-2) which indicates that toxic effects of the substance 6 amino-1,3 naphthalene –disulfonic acid were determined on various fish species such as Leuciscus idus, Brachydanio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate for 96 hrs in static condition and in freshwater. During experiment the lethal concentration (LC0) value was found to be 1000 mg/l. (Chemosphere. Vol. 28, No. 12, pp. 2203.2236, 1994)

 

Thus, based on above lethal concentration values, it can be concluded that the target chemical 5-amino-2-anilinobenzenesulfonic acid (CAS No. 91-30-5) can be considered as non-toxic to fish and thus cannot be classified as hazardous as per the CLP criteria.

Short term toxicity to aquatic invertebrates:

Various predicted studies and read across study for theBenzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5)were reviewed to summarize the following information: 

48 hrs aquatic toxicity studies (SSS QSAR prediction model, 2016) were conducted to assess toxic effects ofBenzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5)and the results were predicted. The study was based on the effects of the test compound on the Daphnia magna in a static fresh water system. The predicted data suggests the effective concentration (EC50) for theBenzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5) wasestimated to be 152.128555298 mg/Lon the basis of intoxication.

48 hrs aquatic toxicity studies(EPI suite, ECOSAR version 1.1, 2016) were conducted to assess toxic effects of theBenzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5) and the results were predicted. The study was based on the effects of the test compound on the Daphnia magna in a static fresh water system. The predicted data suggests the lethal concentration (LC50) forBenzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5) was estimated to be 89374.367mg/l.

Study on read across chemical Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate was performed by abitec laboratory (Determination of the inhibition of the mobility of daphnids ).Determination of the inhibition of the mobility of daphnids was carried out with the substance Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate according to OECD Guideline 202. The test substance was tested at the concentrations 0 and 100 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate, in Daphnia magna was determined to be >100 mg/L for immobilisation effects.This value indicates that the substance is likely to be non-hazardous to aquatic invertebrates and can be classified as "not classified" as per the CLP criteria.

Study on another read across chemical 2-Aminoanthraquinone (117-79-3) which has 50-60% structural similarity short term toxicity to aquatic invertebrates test (Ecotox database, U.S Environmental Protection Agency,2016) was performed in daphnia magna. The experiment period was of 48 hrs. On the basis of intoxication the EC50 value for 2-Aminoanthraquinone was determined to be 378 mg/l. Based on the result the test chemical 2-Aminoanthraquinone (117-79-3) was considered to be not classified as per the CLP regulations.

From results of prediction data of target chemical Benzenesulfonic acid, 5-amino-2-(phenylamino)-,laboratory study of read across chemical Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate (3567-66-6) which is 50-60% structurally similar and study on another read across chemical 2-Aminoanthraquinone (117-79-3) which is 50-60% structurally similar to target chemical suggests that the Benzenesulfonic acid, 5-amino-2-(phenylamino)-was considered to be not hazardous to aquatic invertebrates and can be called to be not classified chemical as per the CLP regulations.

Short-term toxicity to aquatic invertebrates:

Various predicted studies and read across study for Benzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5) were reviewed to summarize the following information: 

48 hrs aquatic toxicity studies (SSS QSAR prediction model, 2016) were conducted to assess toxic effects of Benzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5)and the results were predicted. The study was based on the effects of the test compound on the Daphnia magna in a static fresh water system. The predicted data suggests the effective concentration (EC50) for theBenzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5) wasestimated to be 152.128555298 mg/Lon the basis of intoxication.

48 hrs aquatic toxicity studies(EPI suite, ECOSAR version 1.1, 2016) were conducted to assess toxic effects of theBenzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5) and the results were predicted. The study was based on the effects of the test compound on the Daphnia magna in a static fresh water system. The predicted data suggests the lethal concentration (LC50) forBenzenesulfonic acid, 5-amino-2-(phenylamino)-(91-30-5) was estimated to be 89374.367mg/l.

Study on read across chemical Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate was performed by Abitec laboratory Determination of the inhibition of the mobility of daphnids was carried out with the substance Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate according to OECD Guideline 202. The substance was tested at the concentrations 0 and 100 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate, in Daphnia magna was determined to be >100 mg/L for immobilisation effects.This value indicates that the substance is likely to be non-hazardous to aquatic invertebrates and can be classified as "not classified" as per the CLP criteria.

Study on another read across chemical 2-Aminoanthraquinone (117-79-3) which has 50-60% structural similarity short term toxicity to aquatic invertebrates test (Ecotox database, U.S Environmental Protection Agency,2016) was performed in Daphnia magna. The experiment period was of 48 hrs. On the basis of intoxication the EC50 value for 2-Aminoanthraquinone was determined to be 378 mg/l. Based on the result the test chemical 2-Aminoanthraquinone (117-79-3) was considered to be not classified as per the CLP regulations.

From results of prediction data of target chemical Benzenesulfonic acid, 5-amino-2-(phenylamino)-,laboratory study of read across chemicals Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate (3567-66-6) and 2-Aminoanthraquinone (117-79-3) which are 50-60% structurally similar to target, suggests that the Benzenesulfonic acid, 5-amino-2-(phenylamino)-was considered to be not hazardous to aquatic invertebrates and can be called to be not classified chemical as per the CLP regulations.

Toxicity to aquatic algae and cyanobacteria

Various predicted data for the target chemicalBenzenesulfonic acid, 5-amino-2-(phenylamino)-(CAS No. 91-30-5) and the study for its read across substancewere reviewed to summarize the following information:

 

72 hrs aquatic toxicity study was predicted to assess toxic effects of the test compound Benzenesulfonic acid, 5-amino-2-(phenylamino)- (CAS no. 91 -30 -5) and the result were predicted (SSS QSAR Prediction Model, 2016). The study was based on the effects of the test compound on Desmodesmus subspicatus in a static fresh watersystem. The predicted data suggests the effective concentration (EC50) for the test compound Benzenesulfonic acid, 5-amino-2- (phenylamino)- (CAS no. 91 -30 -5) was estimated to be 138.0 mg/l on the basis of growth rate.

 

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2016), the 96 hours EC50 was estimated to be 18645.9 mg/l on green algae for substance Benzenesulfonic acid, 5-amino-2-(phenylamino)- (CAS no. 91 -30 -5) with growth inhibition effects.

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the read across substance Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate (CAS no. 3567-66-6) according to OECD Guideline 201 (ABITEC study report, Sustainability Support Services (Europe) AB, Report no. 452/16, 2016). The stock solution (200 mg/L) was prepared by dissolving dark red powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of read across chemical used for the study was 12.5, 25, 50, 100, and 200 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the read across substance Disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate in Desmodesmus subspicatus was determined to be 330.2 mg/L on the basis of effects on growth rate in a 72 hour study.

Based on the overall reported results for target and its read across substance, it can be concluded that the test substance Benzenesulfonic acid, 5-amino-2-(phenylamino)- can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Toxicity to microorganisms:

Predicted model data and experimental study results of toxicity to micro organism for the target chemical 5-amino-2-anilinobenzenesulfonic acid (CAS No. 91-30-5) with respective read across were reviewed to summarize the following information:

In key study the 48 hrs aquatic toxicity study was predicted to assess toxic effects of the test compound 5-amino-2-anilinobenzenesulfonic acid (CAS no. 91-30-5) and the result were predicted (SSS QSAR Prediction model, 2016). The study was based on the effects of the test compound on Tetrahymena pyriformis micro organism in a static fresh water system. The predicted data suggests the Inhibition growth concentration (IGC50) for the test compound was estimated to be 681.28717041 mg/l on the basis of growth inhibition effect.     

Whereas the experimental study for read across chemical 4,4'-diaminostilbene-2,2'-disulphonic acid (Cas no. 81-11-8) indicate that the EC0 value of benzidine-2,2'-disulphonic acid in microorganism Pseudomonas fluorescens in 24 hr study to be 1000 mg/L.(HPVIS databse;2016)

Based on above both results the 5-amino-2-anilinobenzenesulfonic acid (CAS No. 91-30-5) was consider to be non toxic to micro organisms.