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Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
16/01/2017 - 19/01/2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guidelines.
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Principles of method if other than guideline:
The effect of Diphenylacetonitrile was evaluated on unicellular green algae
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material (as cited in study report): Diphenylacetonitrile
- Molecular formula: C14H11N
- Molecular weight : 193.248 g/mol
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
not specified
Details on sampling:
The stock solution 100.0 mg/ml was prepared by dissolving white powder in acetone. Test solutions of requiredconcentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
Vehicle:
yes
Details on test solutions:
The stock solution (100 g/L) was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Remarks on exposure duration:
±1 hour
Test temperature:
23 ± 2°C
pH:
without adjustment
The sample concentration 50 mg/l: pH=8.0 (changed to pH=7.5 during the test)
Control: pH=8.1 (changed to pH= 7.7 during the test)
Control + acetone: pH=8.1 (changed to pH= 7.7 during the test)
Nominal and measured concentrations:
0, 3, 6, 12, 24 and 50 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50/ 100 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
12.9 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% CL: 11.5 - 14.6
Results with reference substance (positive control):
Results with reference substance valid
- EC50: 0.69 mg/L
Validity criteria fulfilled:
yes
Conclusions:
The median effective concentration (EC50) for the test substance,diphenylacetonitrile, in Desmodesmus subspicatus was determined to be 12.9 mg/L on the basis of effects on growth rate in a 72 hour study.
Executive summary:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance diphenylacetonitrile, according to OECD Guideline 201.

The stock solution (100 g/L) was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance, diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.

Description of key information

Toxicity to aquatic algae and cyanobacteria:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substancediphenylacetonitrile,according to OECD Guideline 201.

The stock solution (100 g/L) was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:
12.9 mg/L

Additional information

Toxicity to aquatic algae and cyanobacteria:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substancediphenylacetonitrile,according to OECD Guideline 201.

The stock solution (100 g/L) was prepared by dissolving white powder in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 3, 6, 12, 24 and 50 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (EC50) for the test substance,diphenylacetonitrile,,in Desmodesmus subspicatus was determined to be 12.9 mg/L.

This value indicates that the substance is likely to be hazardous to aquatic algae and can be classified as Aquatic Chronic Category 3 as per the CLP criteria.