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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from publication.
Qualifier:
according to
Guideline:
other: refer below principle
Principles of method if other than guideline:
In screening study Observations on the toxic effect of compounds Diphenylacetonitrile on Fingerling of the Oncorhynchus mykiss was noted.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (as cited in study report): Diphenylacetonitrile
- Molecular formula (if other than submission substance): C14H11N
- Molecular weight (if other than submission substance): 193.248 g/mol
- Smiles notation (if other than submission substance): N#CC(c1ccccc1)c1ccccc1
- InChl (if other than submission substance): 1S/C14H11N/c15-11-14(12-7-3-1-4-8-12)13-9-5-2-6-10-13/h1-10,14H
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):2-Propanone or Ethanol
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)):5 PPM
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Source:Ocqueo River , Presque Isle County, Michigan
- Age at study initiation (mean and range, SD):Fingerling
- Length at study initiation (length definition, mean, range and SD):<= 4 inches
-Frequency:Once daily

ACCLIMATION
- Acclimation conditions (same as test or not):Held in running water in aquaria and small "races" under conditions which simulated their natural stream habitat
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
10 h
Hardness:
no data
Test temperature:
55 deg. F.
pH:
7.5 to 8.2
Dissolved oxygen:
8.6 to 13.7 ppm
Salinity:
no data
Nominal and measured concentrations:
Initial test concentration: 5 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:10-liter glass battery Jar
- Type (delete if not applicable): open / closed
- Aeration:aeration through standard stone air - breakers
- Type of flow-through (e.g. peristaltic or proportional diluter):proportional diluter
- Renewal rate of test solution (frequency/flow rate):repeated
- No. of organisms per vessel:6
- No. of vessels per concentration (replicates):12
- No. of vessels per control (replicates):1


Reference substance (positive control):
not specified
Duration:
10 h
Dose descriptor:
LC50
Effect conc.:
5 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
not specified
Conclusions:
Based on the screening study for test compound Diphenylacetonitrile on Fingerling of the Oncorhynchus mykiss (Rainbow Trout) fish the lethal concentration (LC50) was determined to be 5 mg/l on the basis of mortality effect for 10 hrs.
Executive summary:

In screening study Observations on the toxic effect of compounds Diphenylacetonitrile on Fingerling of the Rainbow Trout (Oncorhynchus mykiss) was performed in laboratory under static condition using fresh water. 2-Propanone or Ethanol used as a solvent in 5 ppm concentration at test temperature 55 deg. F. and pH 7.5 to 8.2 with dissolved oxygen 8.6 to 13.7 ppm. During experiment the lethal concentration (LC50) for 10 hrs. was determined to be 5 mg/l on the basis of mortality.

Thus based on this value, it can be concluded that the test chemical can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 2 as per the CLP criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical Diphenyl acetonitrile(86 -29 -3) on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The nominal concentration selected for the experiment were1.39 mg/L,2.09 mg/L,3.14 mg/L,4.72 mg/L,7.08 mg/L.mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

 

The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring.After that analytically detect that stock and set up experimental concentration according to that solubility value i.e, 7.08 mg/L.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

The nominal concentration selected for the experiment was1.39 mg/L,2.09 mg/L,3.14 mg/L,4.72 mg/L,7.08 mg/L.and Zebra fish were exposed to this concentration for 96 hours.The lethal concentrations LC50 was found to be >1.39 mg/L but <2.09 mg/L

After 96 hours of exposure to test item Diphenyl acetonitrile(86 -29 -3) to various nominal test concentrations, LC50 was determine to be>1.39 mg/L but <2.09 mg/Lmg/l . Based on the LC50, it can be consider that the chemical was toxic to fish and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
1.39 mg/L

Additional information

Short term toxicity to fish:

Study report and experimental data was available for short term toxicity of fish of the test chemical Diphenyl acetonitrile(86 -29 -3) The studies are as mentioned below:

1.Study was conducted to access the effect of test chemical Diphenyl acetonitrile(86 -29 -3) on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The nominal concentration selected for the experiment were1.39 mg/L,2.09 mg/L,3.14 mg/L,4.72 mg/L,7.08 mg/L.mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

 

The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 72 hrs continuous stirring.After that analytically detect that stock and set up experimental concentration according to that solubility value i.e, 7.08 mg/L.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.

The nominal concentration selected for the experiment was1.39 mg/L,2.09 mg/L,3.14 mg/L,4.72 mg/L,7.08 mg/L.and Zebra fish were exposed to this concentration for 96 hours.The lethal concentrations LC50 was found to be >1.39 mg/L but <2.09 mg/L

After 96 hours of exposure to test item Diphenyl acetonitrile(86 -29 -3) to various nominal test concentrations, LC50 was determine to be>1.39 mg/L but <2.09 mg/Lmg/l . Based on the LC50, it can be consider that the chemical was toxic to fish and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

2.In screening study Observations on the toxic effect of compounds Diphenylacetonitrile on Fingerling of the Rainbow Trout (Oncorhynchus mykiss) was performed in laboratory under static condition using fresh water. 2-Propanone or Ethanol used as a solvent in 5 ppm concentration at test temperature 55 deg. F. and pH 7.5 to 8.2 with dissolved oxygen 8.6 to 13.7 ppm. During experiment the lethal concentration (LC50) for 10 hrs. was determined to be 5 mg/l on the basis of mortality.

Thus based on this value, it can be concluded that the test chemical can be considered as toxic to aquatic organisms and thuscan be classified as aquatic chronic category 2as per the CLP criteria.

3.In screening study Observations on the toxic effect of compounds Diphenylacetonitrile on Larva(e) of the Petromyzon marinus (Sea Lamprey) was performed in laboratory under static condition using fresh water. 2-Propanone or Ethanol used as a solvent in 5 ppm concentration at test temperature 55 deg. F. and pH 7.5 to 8.2 with dissolved oxygen 8.6 to 13.7 ppm. During experiment the lethal concentration (LC50) for 10 hrs. was determined to be 5 mg/l on the basis of mortality.

Thus based on this value, it can be concluded that the test chemical can be considered as toxic to aquatic organisms and thuscan be classified as aquatic chronic category 2 as per the CLP criteria.

Thus based on this value, it can be concluded that the test chemical can be considered as toxic to aquatic organisms and thuscan be classified as aquatic chronic category 2as per the CLP criteria.