Tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29.1.2016-02.12.2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Experimental test result performed using standard test guideline

Justification for type of information:

Experimental test result performed using standard test guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Principles of method if other than guideline:

Aim of this study was to evaluate the nature of chemical when comes in contact with the test organism. Test was conducted according to the OECD guideline 201.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium

EC Number:

270-648-9

EC Name:

Tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium

Cas Number:

68475-50-3

Molecular formula:

C48H33Al2N9O21S6

IUPAC Name:

dialuminium(3+) tris(5-amino-4-hydroxy-3-[(1E)-2-phenyldiazen-1-yl]naphthalene-2,7-disulfonate)

Constituent 2

Reference substance name:

Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium

IUPAC Name:

Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium

Test material form:

other: Solid

Details on test material:

- Name of test material (as cited in study report): Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium- Molecular formula (if other than submission substance): C48H33Al2N9O21S6- Molecular weight (if other than submission substance): 1318.19 g/mol- SMILES:c12c(cc(c(c2O)\N=N\c2ccccc2)S([O-])(=O)=O)cc(S(=O)(=O)[O-])cc1N.[Al+3].[Al+3].c12c(cc(cc2cc(c(c1O)\N=N\c1ccccc1)S(=O)(=O)[O-])S(=O)(=O)[O-])N.c12c(cc(cc2cc(c(c1O)\N=N\c1ccccc1)S(=O)(=O)[O-])S(=O)(=O)[O-])N- InChI: 1S/3C16H13N3O7S2.2Al/c3*17-12-8-11(27(21,22)23)6-9-7-13(28(24,25)26)15(16(20)14(9)12)19-18-10-4-2-1-3-5-10;;/h3*1-8,20H,17H2,(H,21,22,23)(H,24,25,26);;/q;;;2*+3/p-6/b3*19-18+;;- Substance type: Organic- Physical state: Solid

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Details on test solutions:

The stock solution 322.5 mg/L was prepared by dissolving dark violet powder in OECD growth medium. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with OECD growth medium.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM- Common name: - Strain: 86.81 SAG- Source (laboratory, culture collection): Institute of botany of the ASCR- Initial biomass concentration: 5x10(3) cells /ml - Method of cultivation: No data availableACCLIMATION - No data available- Acclimation period:- Culturing media and conditions (same as test or not):- Any deformed or abnormal cells observed:

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

23±2°C

Nominal and measured concentrations:

10,20,40,80,160 mg/L

Details on test conditions:

TEST SYSTEM- Test vessel:150 ml Glass vessel- Type (delete if not applicable): closed (with air permeable stopper)- Sample volume: -------- ml- Initial cells density: 5x10(3) cells/ml- No. of vessels per concentration (replicates): --------GROWTH MEDIUM- Standard medium used: yesOTHER TEST CONDITIONS- Adjustment of pH: No- Photoperiod: Continuous- Light intensity and quality: 6000-8000 lxEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrations

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

322.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95 % CI

Results with reference substance (positive control):

- Results with reference substance valid- EC50: ----- mg/L (24 hours)

Reported statistics and error estimates:

EC50 was calculated using non linear regression by the software Prism 4.0 OR (The differences in means of control and sample were estimated by the t-test for independent groups at a 95 % confidence level, all individual replicates were used (STATISTICA CZ – data analysis software system, version 9.0, StatSoft, Inc.). Statistically significant differences are for p < 0.05.)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The median effective concentration (ErC50) for the test substance,Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium in Daphnia magna was determined to be 322.5 mg/L on the basis of mobility inhibition effects in a 48 hour study.

Executive summary:

Aim of this study was to assess the short term toxicity ofTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminiumto aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.

 

The stock solution 160 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).-10,20,40,80,160 mg/Lnominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The median effective concentration (EC50) for the test substanceTris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium; tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium, in Daphnia magna was determined to be 322.5 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be classified as per the CLP criteria.