Tris[5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-)]dialuminium

Short-term toxicity to fish

Description of key information

By QSAR Toolbox version 3.3 predicted model the short term toxicity (LC50) in Danio rerio fish was estimated to be 317.83001709 mg/l for 96 hrs in static condition and in fresh water for test substance Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium with mortality effects.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

317.83 mg/L

Additional information

Short term toxicity to fish was assessed for the target compound Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium(CAS no. 68475-50-3) and its related read- across by reviewing a number of studies. The summary of the results are presented below:

 

Short term aquatic toxic effects of the test compound Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium(CAS no 68475-50-3) was predicted (SSS QSAR, 2016). The study was based on the effects of the test compound on the fish species Danio rerio in 96 hrs duration in a fresh water static system. The predicted data suggests the lethal concentration (LC50) for Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium on the basis of mortality to be 317.83001709 mg/L. This value concluded that the test compound does not exhibit short term toxicity to fish Danio rerio and is non hazardous to the aquatic environment.

Predicted model ECOSAR v1.00 indicate the the 96 hours LC50 was estimated to be 7988.025 mg/l on fish for substance Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium with mortality effects.

In Experimental data from UERL laboratory report 2016 for read across disodium 5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate(Cas no. 3567-66-6) 96 h study was conducted on Zebra Fish i.e Danio rerio, at a test concentrations of 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. 

The lethal concentrations LC50 was found to be >100mg/L.

Also UERL laboratory report 2016 for read across 3-[(2, 4-dimethyl-5-sulphonatophenyl) azo]-4-hydroxynaphthalene-1-sulphonate (Cas no. 4548-53-2) indicate that the 96 h study was conducted on Zebra Fish Danio rerio, at a test concentrations of 100 mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. 

Whereas for same read across peer reviewed study from The Journal of Toxicological Science, Vol . 3 205-214, 1978 indicate that the Short term toxicity test was performed on Himegaka(Oryzias latipes)was used and acclimated for 10 days in tap water before experiment. In lab, 1 L solution of pH 7 containing 3,000 mg/litre of a Ponceau SX to be tested. 10 tested fish were kept in the tank without direct sunlight for 48 hrs and survival rate was determined.

 From the experiment, TLm (LC 50) value for the short term toxicity to fish was found to be greater than 3000mg/kg.

Whereas experimental study (Chemophere. Vol. 28, No. 12, pp. 2203.2236, 1994) for read across substance 6-aminonaphthalene-1,3-disulphonic acid (118-33-2)indicate that the lethal concentration (LC0) of test substance for fish Leuciscus idus, Bruchydunio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate was found to be 1000 mg/l for 96 hrs.

And other database (Data bank of Environmental Properties of Chemicals (EnviChem), 2014) for read across Sodium naphthionate (Cas no.130-13-2)indicate that the median lethal concentration (LC50) of test substance substance Sodium naphthionate for fish Oryzias latipes was found to be >4000 mg/l for 48 hrs. on the basis of mortality effects.

For read across 2-aminonaphthalene-1-sulphonic acid (Cas no. 81-16-3) from Data bank of Environmental Properties of Chemicals (EnviChem), 2014 indicate the lethal concentration (LC50) for test substance 2 Naphthylamine sulfonic acid on fish species Oryzias latipes was found to be 610 mg/l.

 

Based on above available studies for target chemical and its read across it is concluded that the substance Tris(5-amino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonato(2-))dialuminium(CAS no. 68475-50-3) not qualify for the aquatic classification as per the CLP criteria.