Geranyl butyrate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Study of artificial flavouring substances for mutagenicity in the Salmonella/microsome, Basc and micronucleus tests

Author:

D. Wild, M.-T. King, E. Gocke and K. Eckhardt

Year:

1983

Bibliographic source:

Fd Chem. Toxic. Vol. 21, No. 6, pp. 707-719, 1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Standard plate procedure (Ames, McCann & Yamasaki, 1975)

Principles of method if other than guideline:

Gene mutation toxicity was performed to determine the mutagenic nature of Allyl capronate

GLP compliance:

not specified

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Allyl capronate

IUPAC Name:

Allyl capronate

Constituent 2

Reference substance name:

Allyl hexanoate

EC Number:

204-642-4

EC Name:

Allyl hexanoate

Cas Number:

123-68-2

IUPAC Name:

allyl hexanoate

Details on test material:

- Name of test material: Allyl capronate (Allyl hexanoate)
- Molecular formula: C9H16O2
- Molecular weight: 156.223 g/mol
- Substance type: organic
- Physical state: No data available
- Purity: No data available
- Impurities (identity and concentrations): No data available

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

S-9 liver fractions were prepared from Aroclor-pretreated rats (Aroclor 1254, 500 mg/kg ip) and adjusted to 25 mg protein/ml

Test concentrations with justification for top dose:

5 doses of the test substance were used upto 3600 µg/plate

Vehicle / solvent:

DMSO (if the material was poorly insoluble in water)/ water

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

sodium azide

benzo(a)pyrene

Details on test system and experimental conditions:

METHOD OF APPLICATION: Plate incorporation method

DURATION
- Preincubation period: No data available
- Exposure duration: 48 hr
- Expression time (cells in growth medium): 48 hr
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available

SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available

NUMBER OF REPLICATIONS: Duplicate

NUMBER OF CELLS EVALUATED: No data available

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available

OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available

OTHER: No data available

Evaluation criteria:

A reproducible, dose-related and at least two-fold elevation of the spontaneous revertant frequency. Agents producing reproducible, dose-related and significant (P≤ 0.01) but less than two-fold elevations were classified as marginally mutagenic under the experimental conditions.

Statistics:

Statistical significance was determined according to the methods of Kastenbaum & Bowman (1970).

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

No data available

Remarks on result:

other: strain/cell type:

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with and without

Allyl capronate failed to induce mutation in Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, TA1538 in the presence and absence of S9 metabolic activation system and hence is not likely to classify as a gene mutant in vitro.

Executive summary:

Gene mutation toxicity was performed to determine the mutagenic nature of Allyl capronate.

 

The test was performed by plate incorporation method using Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, TA1538 with and without S9 metabolic activation system at 5 doses of the test substance were used upto 3600 µg/plate and the plates were incubated for 48hrs.

 

Allyl capronate failed to induce mutation in Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, TA1538 in the presenceand absence of S9 metabolic activation system and hence is not likely to classify as a gene mutant in vitro.