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EC number: 249-276-6 | CAS number: 28872-01-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 02 February 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles. Read accross used from oleyl dipropylene triamine.
- Justification for type of information:
- (See also Category polyamines in support of Oleyl dipropylenetriamine attached to Ch.13)
For the polyamines, the substance which was considered likely to have the least corrosive properties was selected for an in vivo confirmatory study. As experience (eg. from primary fatty amines, but also indicated by comparing cytotoxicity scores from genotoxicity studies with the various polyamines) indicate that corrosivity diminishes with increasing alkyl chain length, the substance oleyl tripropylene tetramine was selected, as this has the largest alkyl chain.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2500 (Acute Dermal Irritation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Japanese Ministry of Agriculture, Forestry and Fisheries (JMAFF), 12 Nousan, Notification No 8147, November 2000, including the most recent partial revisions.
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Amines, N-(3- aminopropyl)-N’-[3-(C18 and C18-unsatd. alkyl amino)propyl]trimethylenedi and amines, N-(3-aminopropyl)-N’-(C18 and C18-unsatd. alkyl)trimethylenedi-
- EC Number:
- 701-369-7
- Cas Number:
- not available
- Molecular formula:
- UVCB - No molecular formula;
- IUPAC Name:
- Amines, N-(3- aminopropyl)-N’-[3-(C18 and C18-unsatd. alkyl amino)propyl]trimethylenedi and amines, N-(3-aminopropyl)-N’-(C18 and C18-unsatd. alkyl)trimethylenedi-
- Reference substance name:
- (Z)-N-(3-aminopropyl)-N'-[3-(9-octadecenylamino)propyl]propane-1,3-diamine
- EC Number:
- 266-613-2
- EC Name:
- (Z)-N-(3-aminopropyl)-N'-[3-(9-octadecenylamino)propyl]propane-1,3-diamine
- Cas Number:
- 67228-83-5
- Molecular formula:
- C27H58N4
- IUPAC Name:
- N-(3-aminopropyl)-N'-[3-(octadec-9-en-1-ylamino)propyl]propane-1,3-diamine
- Reference substance name:
- Oleyl tripropylenetetraamine.
- IUPAC Name:
- Oleyl tripropylenetetraamine.
- Reference substance name:
- Tetraamine O
- IUPAC Name:
- Tetraamine O
- Details on test material:
- - Name of test material (as cited in study report): Oleyl tripropylenetetramine
- Old EC No.: 266-613-2
- EC No.: 701-369-7
- Substance type: white paste
- Physical state: solid
- Analytical purity: see attached certificate of analysis
- Impurities (identity and concentrations): Free acrylonitrile <4 ppm, water: 0.1 %)
- Composition of test material, percentage of components: see attached certificate of analysis
- Purity test date: 5 February 2009
- Lot/batch No.:S001029
- Expiration date of the lot/batch: 14 May 2017
- Stability under test conditions: stable
- Storage condition of test material: At room temperature in the dark under Nitrogen
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan, Belton, Leics, England.
- Age at study initiation: at least 6 weeks
- Weight at study initiation: at least 1.0 kg
- Housing: The animal was individually housed in a labeled cage with perforated floor and shelter
- Diet: ad libitum
- Water: ad libitum)
- Acclimation period: Acclimatization period was at least 5 days before start of treatment under laboratory conditions
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18.8 - 20.6ºC
- Humidity (%): 44 - 74%
- Air changes (per hr): approximately 15
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: 02 February 2010
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 grams
pH (1% in water, indicative range): 10.2-10.7 - Duration of treatment / exposure:
- 3 minutes, 1 hour and 4 hours:
The study was initiated by treatment of one rabbit. This animal received of 0.5 grams of the test substance to the intact, clipped skin of one flank using a Metalline patch of 2x3 cm. The patch was mounted on Micropore tape#, which was wrapped around the abdomen and secured with Coban elastic bandage. The dressing was removed 3 minutes after application. Since no signs of severe skin reactions (i.e. necrosis or corrosion) were observed and it was considered that exposure could be continued humanely, two samples of 0.5 grams of the test substance were then applied to separate skin-sites on the intact, clipped skin of the same animal, using an identical procedure and one sample per dressing. One of the dressings was removed after a 1-hour exposure. After similar considerations (i.e. no severe skin reactions, necrosis or corrosion), the other dressing was removed after a 4-hour exposure. Since signs of severe skin reactions (i.e. necrosis) were observed after 4 hours of exposure, the study was immediately terminated, and no further testing was performed. - Observation period:
- up to 4 hours after the first application when the single treated animal was sacrificed for ethical reasons.
- Number of animals:
- 1 (based on the severe skin reactions, no further animals were exposed to the test substance)
- Details on study design:
- TEST SITE
- Area of exposure: 2x3 cm
- Type of wrap if used: Coban elastic bandage
REMOVAL OF TEST SUBSTANCE
- Washing (if done): water and ethanol
- Time after start of exposure: immediately
SCORING SYSTEM:
The skin reactions of all visible treated sites were assessed immediately after removal of a dressing. The irritation scores and a description of all other (local) effects were recorded. Adjacent areas of untreated skin of the animal served as controls.
The irritation was assessed according to the following numerical scoring system. At each observation, the highest scores given were recorded:
Erythema and eschar formation:
No erythema 0
Very slight erythema (barely perceptible) 1
Well-defined erythema 2
Moderate to severe erythema 3
Severe erythema (beef redness) * 4
*. Where signs of necrosis or corrosion (injuries in depth) prevent erythema scoring, the
maximum grade for erythema (= 4) is given.
Oedema formation:
No oedema 0
Very slight oedema (barely perceptible) 1
Slight oedema (edges of area well-defined by definite raising) 2
Moderate oedema (raised approximately 1 millimeter) 3
Severe oedema (raised more than 1 millimeter and extending beyond the area of exposure) 4
Other observations:
Mortality/Viability Twice daily.
Toxicity At least once daily.
Body Weight Day of treatment (prior to application).
Necropsy No necropsy was performed.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Remarks on result:
- other: not reported
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 4
- Max. score:
- 4
- Reversibility:
- other: Dark-brown discolouration surrounded by grey discolouration at the edges of the application area, a sign of necrosis.
- Remarks on result:
- other: Considered corrosive. Study terminated. Immediately after 4 hr exposure
- Irritant / corrosive response data:
- A 3-minute exposure to 0.5 g of Oleyl tripropylenetetramine resulted in very slight erythema at 1 and 4 hours after exposure.
A 1-hour exposure resulted in very slight erythema immediately after exposure and in well defined erythema at 3 hours after exposure.
A 4-hour exposure resulted in dark brown discolouration surrounded by grey discolouration at the edge of the application area (sign of necrosis) immediately after exposure. - Other effects:
- Sticky or dry remnants of the test substance were present on the skin after exposure.
Any other information on results incl. tables
See attached table Individual irritation scores.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1C (corrosive) based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- Oleyl tripropylenetetramine should be classified as : skin corrosive (Category 1C).
- Executive summary:
One rabbit was exposed to three samples of 0.5 grams of Oleyl tripropylenetetramine applied to separate skin-sites on intact, clipped skin using a semi-occlusive dressing. The exposure periods were 3 minutes, 1 hour and 4 hours, respectively. Skin reactions were assessed up to 4 hours after the 3-minute exposure. Based on severe skin reactions, no further animals were exposed to the test substance.
A 3-minute exposure to 0.5 g of Oleyl tripropylenetetramine resulted in very slight erythema at 1 and 4 hours after exposure.
A 1-hour exposure resulted in very slight erythema immediately after exposure and in well defined erythema at 3 hours after exposure.
A 4-hour exposure resulted in dark brown discolouration surrounded by grey discolouration at the edge of the application area (sign of necrosis) immediately after exposure.
Sticky or dry remnants of the test substance were present on the skin after exposure.
The dark brown discolouration surrounded by grey discolouration at the edge of the application area are signs of necrosis. Following this observation, the animal was sacrificed for humane reasons. These severe skin reactions are expected to result in deep and thick scab formation with possible ruptures of the scab, or the scab may drop off exposing scar tissue. Overall, these skin reactions were considered evidence of full thickness destruction of the skin, and hence no further animals were tested.
Based on these results and according to the:
- Globally Harmonized System of Classification and Labeling of Chemicals (GHS) of the United Nations (2007), Oleyl tripropylenetetramine should be classified as : skin corrosive (Category 1C).
- Regulation (EC) No 1272/2008 on classification, labeling and packaging of substances and mixtures, Oleyl tripropylenetetramine should be classified as Corrosive (Category 1C) and labeled as H314: Causes severe skin burns and eye damage.
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