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No short-term terrestrial test data is available for the alkyl polypropylenepolyamines. One long-term terrestrial test is performed with alkyl polypropylenepolyamines. The effects of tallowalkyl l polypropylene polyamines on mortality, biomass and reproduction of Eisenia fetida was tested according to OECD 222 under a static exposure for 28 days. Natural soil (Lufa 2.2) was used as substrate, and the different concentrations of the substance mixed with the substrate. The concentrations applied were 62.5, 125, 250, 500, and 1,000 mg/kg soil dry weight. In addition tests using control were performed.

The test is valid without restrictions and the results are reliable. After 28 days of exposure, no effects on survival of the adult worms were observed in all concentrations. After the following four weeks, no significant effects on the reproduction rate (average number of juveniles) were observed compared to the control. Hence, the NOEC is 1000 mg a.i./kg dw soil.