Isopentyl phenethyl ether

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

22 February to 5 April 1982

Reliability:

4 (not assignable)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Oral intubation for 4 - 5 week old white mice.
Groups of mice were intubated at 3 dose levels using graded volumes of the test substance. Animals were observed for up to 7 days after the intubation. All animals dying were autopsied. All survivors were killed and examined post mortem after 7 days.

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isopentyl phenethyl ether

EC Number:

259-943-3

EC Name:

Isopentyl phenethyl ether

Cas Number:

56011-02-0

Molecular formula:

C13H20O

IUPAC Name:

isopentyl phenethyl ether

Specific details on test material used for the study:

material name (as stated in the report): ANTHER
Appearance: Clear colourless liquid

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

The animals are placed in individual cages, fasted for hours and then the animals were individually weighed.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

Animals were intubated with the appropriate volumes of the test material. Each

Doses:

10, 5 and 2 mg/Kg Body weight

No. of animals per sex per dose:

Each animal on one dosage level receives the same amount per Kg body weight.

Control animals:

no

Details on study design:

Groups of mice were intubated at 3 dose levels using graded volumes of the test substance. Animals were observed for up to 7 days after the intubation. All animals dying were autopsied. All survivors were killed and examined post mortem after 7 days.

Results and discussion

Effect levels

Key result

Sex:

not specified

Dose descriptor:

LD50

Effect level:

5 000 mg/kg bw

Based on:

test mat.

Mortality:

10 mg/Kg Body weight leads to 2 deaths (2 animals treated)
5 mg/Kg Body weight leads to 3 deaths (6 animals treated)
2 mg/Kg Body weight leads to 0 death (2 animals treated)

Clinical signs:

other: 10 and 5 mg/Kg Body weight : signs of stress, somnolent, hypothermy and laboured breating

Any other information on results incl. tables

All mice dosed at 10 and 5 mg/Kg Body weight were showing signs of stress within 1 hour after treatment. The mice dosed at 10 mg/Kg bw became somnolent and died within 18 hours.

The mice dosed at 5 mg/Kg Body weight also became somnolent and two mice died within 18 hours. At this time, these mice were hypothermic and showing laboured breathing and one more mouse died within 90 hours. The surviving mice recovered within 48 hours.

The mice dosed at 2 mg/Kg bw appeared unaffected by the treatment.

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

The LD50 (oral) of ANTHER was determined to be 5000 mg/Kg Body weight according to this test. Therefore it can be concluded that the test substance ANTHER does not meet the criteria to be classified according to the CLP Regulation 1272/2008/EC.

Executive summary:

The LD50 (oral) of Anther was determined in this Acute Oral Toxicity Test dated on 4 April 1982, performed at Unilever research Laboratory.

Groups of mice were intubated at 3 dose levels using graded volumes of the test substance. Animals were observed for up to 7 days after intubation. All animals dying were autopsied. All survivors were killed and examined post mortem after 7 days.

All mice dosed at 10 and 5 mg/Kg Body weight were showing signs of stress within 1 hour after treatment. The mice dosed at 10 mg/Kg bw became somnolent and died within 18 hours.

The mice dosed at 5 mg/Kg Body weight also became somnolent and two mice died within 18 hours. At this time, these mice were hypothermic and showing laboured breathing and one more mouse died within 90 hours. The surviving mice recovered within 48 hours. The mice dosed at 2 mg/Kg bw appeared unaffected by the treatment. The LD50 (oral) of ANTHER was determined to be 5000 mg/Kg Body weight according to this test. Therefore it can be concluded that the test substance ANTHER does not meet the criteria to be classified according to the CLP Regulation 1272/2008/EC, but does according to GHS criteria (Cat.5.) based on 3 out of 6 animals dying at 5 g/kg.