4',5'-dibromo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Data source

Reference

Reference Type:

publication

Title:

Toxicity of Rhodamine B and Fluorescein Sodium to Fish and their Compatibility with Antimycin A.

Author:

Leif L. Marking

Year:

1969

Bibliographic source:

The Progressive Fish – Culturist, Vol. 31 (3), Pg. no. 139-142, 1969.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Short term toxicity study to Rainbow trout (Salmo gairdneri), Channel catfish (Ictalurus punctatus) and Bluegill (Lepomis macrochirus) was carried out for 96 hrs.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate

EC Number:

208-253-0

EC Name:

Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate

Cas Number:

518-47-8

Molecular formula:

C20H12O5.2Na

IUPAC Name:

disodium 3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate

Test material form:

other: Orange reddish powder

Details on test material:

- Name of test material (as cited in study report): Fluorescein sodium - Molecular formula (if other than submission substance): C20-H10-O5.2Na- Molecular weight (if other than submission substance): 376.274 g/mol- Smiles notation (if other than submission substance): c1(c2c(oc3c1ccc(c3)[O-])cc(=O)cc2)c1c(cccc1)C(=O)[O-].[Na+].[Na+]- InChl (if other than submission substance): 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;;/h1-10,21H,(H,23,24);;/q;2*+1/p-2- Substance type: Organic - Physical state: Solid

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.- Sampling method: The dye fluorescein sodium (9-(0-carboxyphenyl)-6-hydroxy-3-isoxanthenone) was acquired from Matheson, Coleman, and Bell in a dry powder in a grade.The dye for the toxicity study was weighed separately for each vessel.Stock solutions of the water-soluble dye was prepared for concentrations of 5 and 100 p.p.m. in the antimycin-dye mixtures. Because antimycin is relatively insoluble in water, it was first dissolved in acetone and then diluted with water.

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

other: 3 different test organisms were used for the study. The test organism include- 1.Rainbow trout (Salmo gairdneri) 2.Channel catfish (Ictalurus punctatus) and 3.Bluegill (Lepomis macrochirus)

Details on test organisms:

No data available

Study design

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

LC50 value was determined at 24, 48 and 96 hrs.

Test conditions

Hardness:

No data available

Test temperature:

12°C

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Nominal and measured concentrations:

Nominal concentrations

Details on test conditions:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): LC50 value was determined at 24, 48 and 96 hrs.TEST CONCENTRATIONS- Test concentrations: The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

4 198 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

3 420 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 372 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Rainbow trout.

Effect concentrations 4

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

3 828 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.

Effect concentrations 5

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

2 826 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2 267 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Channel catfish.

Effect concentrations 7

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.

Effect concentrations 8

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4 898 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.

Effect concentrations 9

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3 433 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence interval, the LC50 vaue is for test animal Bluegill.

Reported statistics and error estimates:

No data available

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table: Toxicity of fluorescein sodium to test fishes at 12°C.

 

Dye	Species	LC50 and 95% confidence interval (ppm) at:
		24 hrs	48 hrs	96 hrs
Fluorescein sodium	Rainbow trout     Channel catfish     Bluegill	4,198 (3,695 – 4,763)   3,828 (3,560 – 4,115)   5,000 (4,308 – 5,800)	3,420 (3,000 -3,900)   2,826 (2,559 – 3,123)   4,898 (4,153 – 5,765)	1,372 (1,023 – 1,840)   2,267 (1,928 – 2,670)   3,433 (2,922 – 4,028)

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-Rainbow trout: LC50 values at24 hrs - 4198 mg/l48 hrs - 3420 mg/l96 hrs –1372 mg/lChannel catfish: LC50 values at24 hrs -3828 mg/l48 hrs -2826 mg/l96 hrs –2267 mg/lBluegill: LC50 values at24 hrs -5000 mg/l48 hrs -4898 mg/l96 hrs –3433 mg/l

Executive summary:

Short term toxicity study to 3 different species of fish was carried out for 96 hrs.The test organism include-

1.      Rainbow trout (Salmo gairdneri)

2.      Channel catfish (Ictalurus punctatus) and

3.      Bluegill (Lepomis macrochirus)

 

The dye fluorescein sodium (9-(0-carboxyphenyl)-6-hydroxy-3-isoxanthenone) was acquired from Matheson, Coleman, and Bell in a dry powder in a grade.

 

The dye for the toxicity study was weighed separately for each vessel.

 

Stock solutions of the water-soluble dye was prepared for concentrations of 5 and 100 p.p.m. in the antimycin-dye mixtures. Because antimycin is relatively insoluble in water, it was first dissolved in acetone and then diluted with water.

 

The conc. of test chemical used for the study was 5 and 100 mg/l (5 and 100 ppm), respectively.

 

The toxicity study was performed at temperature of 21°C. LC50 value was determined at 24, 48 and 96 hrs.

 

Based on mortality of test organism Rainbow trout, Channel catfish and Bluegill, the 24, 48 and 96 hrs LC50 values was given-

 

Rainbow trout:LC50 values at

24 hrs - 4198 mg/l

48 hrs - 3420 mg/l

96 hrs –1372 mg/l

 

Channel catfish:LC50 values at

24 hrs -3828 mg/l

48 hrs -2826 mg/l

96 hrs –2267 mg/l

 

Bluegill:LC50 values at

24 hrs -5000 mg/l

48 hrs -4898 mg/l

96 hrs –3433 mg/l