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EC number: 944-243-9 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 Apr - 13 May 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal wastewater treatment plant Breisgauer Bucht- Preparation of inoculum for exposure: washed twice by settling the sludge, decanting the supernatant and resuspending the sludge in aerated tap water- Concentration of sludge: 30 mg dry solids/L
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS- Composition of medium: according to guideline- Test temperature: 21.0-21.5 °C- Suspended solids concentration: 30 mg/L dry solids- Other: constant stirring using magnetic stirrersTEST SYSTEM- Culturing apparatus: 2L gas wash bottles- Number of culture flasks/concentration: 2- Method used to create aerobic conditions: aerated with CO2-free air at a rate of 50-100 mL/min overnight- Measuring equipment: IC determination- Details of trap for CO2 and volatile organics if used: 0.2M NaOHSAMPLING- Sampling frequency: Day 0, 4, 7, 11, 14, 21 and 28- Sampling method: Absorber flask exchangedCONTROL AND BLANK SYSTEM- Inoculum blank: 2- Toxicity control: 1- Reference control: 1
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 95
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 81.5
- Sampling time:
- 10 d
- Details on results:
- The 10-day window was fulfilled.The degradation in the toxicity control was 91.2% within 14 days and 95.8% within 28 days.
- Results with reference substance:
- Sodium benzoate reached the pass levels for ready biodegradability within 4 days.
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal wastewater treatment plant Breisgauer Bucht- Preparation of inoculum for exposure: washed twice by settling the sludge, decanting the supernatant and resuspending the sludge in aerated tap water- Concentration of sludge: 30 mg dry solids/L
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS- Composition of medium: according to guideline- Test temperature: 21.0-21.5 °C- Suspended solids concentration: 30 mg/L dry solids- Other: constant stirring using magnetic stirrersTEST SYSTEM- Culturing apparatus: 2L gas wash bottles- Number of culture flasks/concentration: 2- Method used to create aerobic conditions: aerated with CO2-free air at a rate of 50-100 mL/min overnight- Measuring equipment: IC determination- Details of trap for CO2 and volatile organics if used: 0.2M NaOHSAMPLING- Sampling frequency: Day 0, 4, 7, 11, 14, 21 and 28- Sampling method: Absorber flask exchangedCONTROL AND BLANK SYSTEM- Inoculum blank: 2- Toxicity control: 1- Reference control: 1
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 95
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 81.5
- Sampling time:
- 10 d
- Details on results:
- The 10-day window was fulfilled.The degradation in the toxicity control was 91.2% within 14 days and 95.8% within 28 days.
- Results with reference substance:
- Sodium benzoate reached the pass levels for ready biodegradability within 4 days.
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions.
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: effluent of the sewage treatment plant Hochdahl which treats predominantly domestic sewage.
- Duration of test (contact time):
- 30 d
- Initial conc.:
- 2 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 5 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- other: theoretical oxygen demand
- Details on study design:
- TEST CONDITIONS- Composition of medium: deionised water- Aeration of dilution water: yes, saturation 8-9 mg oxygen/LCONTROL AND BLANK SYSTEM- sodium decyl sulphate was used as a reference substance
- Reference substance:
- other: C12AS-Na (sodium decyl sulphate) 2 mg/L
- Parameter:
- other: chemical oxygen demand
- Value:
- 98
- Sampling time:
- 30 d
- Remarks on result:
- other: at concentration 2 mg/L
- Parameter:
- other: chemical oxygen demand
- Value:
- 92
- Sampling time:
- 30 d
- Remarks on result:
- other: at concentration 5 mg/L
- Details on results:
- Kinetic of test substance (2 mg/L): 63% after 5 days99% after 15 days98% after 30 daysKinetic of test substance (5 mg/L): 69% after 5 days 94% after 15 days92% after 30 daysThe test substance was biodegraded by > 60% within the 10-day time window. Thus, it can be considered as readily biodegradable.
- Results with reference substance:
- Kinetic of the reference substance:42% after 5 days64% after 15 days63% after 30 days
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions.
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Duration of test (contact time):
- 8 d
- Initial conc.:
- 75 mg/L
- Based on:
- test mat.
- Value:
- ca. 90
- Sampling time:
- 8 d
- Remarks on result:
- other: value taken from a graphic
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: effluent of domestic sewage treatment plant
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Sewage treatment plant Hochdahl, municipal treatment plant
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 5 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 97
- Sampling time:
- 28 d
- Remarks on result:
- other: 2 mg/L
- Parameter:
- % degradation (O2 consumption)
- Value:
- 94
- Sampling time:
- 28 d
- Remarks on result:
- other: 5 mg/L
- Results with reference substance:
- 84% degradation of sodium benzoate after 28 days.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: effluent of the sewage treatment plant Hochdahl which treats predominantly domestic sewage.
- Duration of test (contact time):
- 30 d
- Initial conc.:
- 2 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 5 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- other: theoretical oxygen demand
- Details on study design:
- TEST CONDITIONS- Composition of medium: deionised water- Aeration of dilution water: yes, saturation 8-9 mg oxygen/LCONTROL AND BLANK SYSTEM- sodium decyl sulphate was used as a reference substance
- Reference substance:
- other: C12AS-Na (sodium decyl sulphate) 2 mg/L
- Parameter:
- other: chemical oxygen demand
- Value:
- 98
- Sampling time:
- 30 d
- Details on results:
- Kinetic of test substance (2 mg/L): 63% after 5 days99% after 15 days98% after 30 daysKinetic of test substance (5 mg/L): 69% after 5 days 94% after 15 days92% after 30 daysThe test substance was biodegraded by > 60% within the 10-day time window. Thus, it can be considered as readily biodegradable.
- Results with reference substance:
- Kinetic of the reference substance:42% after 5 days64% after 15 days63% after 30 days
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Oxygen conditions:
- aerobic
- Duration of test (contact time):
- 8 d
- Initial conc.:
- 75 mg/L
- Based on:
- test mat.
- Value:
- ca. 90
- Sampling time:
- 8 d
- Remarks on result:
- other: value taken from a graphic
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Refer to the Category Approach Justification document provided in IUCLID6 Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: effluent of domestic sewage treatment plant
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Sewage treatment plant Hochdahl, municipal treatment plant
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 5 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 97
- Sampling time:
- 28 d
- Remarks on result:
- other: 2 mg/L
- Parameter:
- % degradation (O2 consumption)
- Value:
- 94
- Sampling time:
- 28 d
- Remarks on result:
- other: 5 mg/L
- Results with reference substance:
- 84% degradation of sodium benzoate after 28 days.
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
Description of key information
Readily biodegradable according to OECD criteria: 95% after 28 days based on CO2 evolution (OECD 301 B).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Since no reliable ready biodegradability tests are available for C9-12AS Na, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5, a read-across to other structurally related category members was conducted. Read-across data on the following substances were used: C10AS Na (CAS 142-87-0) and C12AS Na (CAS 151-21-3).
The key study with C12AS Na (CAS 151-21-3) was performed according to OECD 301 B and is GLP compliant (Elementis, 2005). Non-adapted activated sludge coming from a municipal wastewater treatment plant was used as inoculum and the initial concentration of the test substance was 20 mg/L as DOC. Biodegradation measured as CO2 evolution reached 95% after 28 days and passed a 10-day window (81.5% after 10 days).
A supporting study with C12AS Na (CAS 151-21-3) was performed according to OECD guideline 301 D and GLP (BASF, 1996). Effluent of domestic sewage treatment plant was used as inoculum and the initial concentrations of the test substance were 2 mg/L and 5 mg/L. Biodegradation after 28 days measured as O2 consumption reached 97% at the concentration of 2 mg/L and 94% at the concentration of 5 mg/L. It can be concluded that C12AS Na (CAS 151-21-3) is ready biodegradable according to OECD criteria.
Two further supporting studies are available for C10AS Na (CAS 142-87-0). One non-GLP study assessing biodegradation of C10AS Na (CAS 142-87-0) was performed according to OECD guideline 301 D (BASF, 2001). Effluent of the sewage treatment plant handling predominantly domestic sewage was used as inoculum. The initial concentrations of the test substance were 2 mg/L and 5 mg/L. Biodegradation of 98% measured as oxygen demand was observed after 30 days at the initial concentration of 2 mg/L and 92% at the concentration of 5 mg/L. The test substance was biodegraded by more than 60% in both cases within five days. Based on these results it can be concluded that C10AS Na (CAS 142-87-0) is readily biodegradable. This result was confirmed by a supporting non-GLP study performed according to OECD guideline 301 E (Sanchez Leal et al., 1991). Biodegradation reached ca. 90% after 8 days of testing.
Due to structural and property similarities with the tested category member these results also apply to C9-12AS Na. This substance is considered to be readily biodegradable according to OECD criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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