2,2,2-trifluoroethyl methacrylate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test), 4 April 1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test), 17 July 1992

Deviations:

no

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

other: no data

Details on inoculum:

- no data

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

28 d

Nominal and measured concentrations:

3 mg/L

Details on test conditions:

TEST SYSTEM
- Number of culture flasks/concentration: 3 (test substance); 2 (inoculum blank, toxicity control, reference substance)
- Test performed in closed vessels due to significant volatility of test substance: closed bottle test

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes; medium + inoculum
- Abiotic sterile control: no
- Toxicity control: yes; medium + inoculum + test item (3 mg/L) + reference substance (2 mg/L)
- Reference substance: medium + inoculum + reference substance (4 mg/L)

Reference substance (positive control):

no

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The biodegradation reached app. 52% 10 days after the beginning of biodegradation (10-d window). After 21 d a plateau was reached at app. 54.4% degradation.
The reference substance (Sodium benzoate) degraded normally under the test conditions (80.1% after 14 d).
The biodegradation in the toxicity control reached 68.2 (day 7) to 73.3% (day 28). The test substance showed no inhibitory effects to microorganisms.

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of TFMEA to microorganisms was investigated during a ready biodegradation study. According to the criteria of the OECD guideline 301 D a substance can be assumed to be inhibitory, if less than 25% degradation occurred within 14 days. The test item showed no inhibitory effects to microorganisms at 3 mg/L.

Executive summary:

The toxicity of TFMEA to microorganisms was investigated during a ready biodegradation study according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test), adopted 17 July 1992 and EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test), over a period of 28 days. The type of inoculum was not specified in the study report. The biodegradation rate was determined by measurement of O2 consumption. Inoculum blank and procedural/functional control with the reference substance sodium benzoate were performed.

The functional control reached the pass level >60% after 7 d. The biodegradation of the test substance reached 52% 10 days after the beginning of biodegradation (10 d window). After 21 d, a plateau was reached at 54.4% degradation. The pass level of 60% was not reached within the test period.

The biodegradation in the toxicity control reached 68.2 (day 7) to 73.3% (day 28).

According to the criteria of the OECD guideline 301 D a substance can be assumed to be inhibitory, if less than 25% degradation occurred within 14 days. The test substance showed no inhibitory effects to microorganisms at a concentration of 3 mg/L.

Description of key information

NOEC = 3 mg/L; OECD guideline 301 D, toxicity control; non-GLP

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

3 mg/L

Additional information

One toxicity screening study conducted during the OECD 301 D biodegradation test is available for TFMEA, which is considered reliable with restriction (Klimisch score 2).

The toxicity of TFMEA to microorganisms was investigated during a ready biodegradation study according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test), adopted 17 July 1992 and EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test), over a period of 28 days. The type of inoculum was not specified in the study report. The biodegradation rate was determined by measurement of O2 consumption. Inoculum blank and procedural/functional control with the reference substance sodium benzoate were performed.

The functional control reached the pass level >60% after 7 d. The biodegradation of the test substance reached 52% 10 days after the beginning of biodegradation (10 d window). After 21 d, a plateau was reached at 54.4% degradation. The pass level of 60% was not reached within the test period.

The biodegradation in the toxicity control reached 68.2 (day 7) to 73.3% (day 28).

According to the criteria of the OECD guideline 301 D a substance can be assumed to be inhibitory, if less than 25% degradation occurred within 14 days. The test substance showed no inhibitory effects to microorganisms at a concentration of 3 mg/L.