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Freshwater aquatic toxicity studies with TFMEA were conducted in invertebrates, algal and microbial species.

Short-term toxicity to aquatic invertebrates

The acute toxicity of TFMEA to Daphnia magna was studied under static conditions according to OECD Guideline 202, adopted 4 April 2008, and EU test method C.2, 29 December 1992. Daphnids were exposed to control, and test chemical at initial concentrations of <0.8, 7.8, 7.7, 9.2, 15.2, 19, 30.1, 40.7, 64.1 mg/L.

The 48 h EC50 based on immobilisation was 19 mg/L (95% CL = 16 - 24 mg/L).

Toxicity to aquatic algae

In a 72 hour acute toxicity study, the cultures of Desmodesmus subspicatus CHODAT SAG 86.81 were exposed to TFMEA at concentrations of nominal 1.00, 3.16, 10.00, 31.60, 100% saturated solution, corresponding to measured (geometric mean) 1.54, 4.95, 18.8, 60.7 and 197 mg/L under static conditions in accordance with the OECD guideline 201 (adopted 23 March 2006).  

Microscopic evaluation of the cells at start and end of the incubation period revealed no morphological abnormalities.

The NOEC-values, EC10-values and EC50-values for inhibition of growth rate after 72 hours were 18.8 and 1.54 mg/L, 23.1 (18.4-33.7) and 39.5 (32.2 - 45.1), respectively. All effect levels are given based on geometric mean measured concentrations of TFMEA.

Toxicity to microorganisms

The toxicity of TFMEA to microorganisms was investigated during a ready biodegradation study according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test), adopted 17 July 1992 and EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test), over a period of 28 days. The type of inoculum was not specified in the study report. The biodegradation rate was determined by measurement of O2 consumption. Inoculum blank and procedural/functional control with the reference substance sodium benzoate were performed.

The functional control reached the pass level >60% after 7 d. The biodegradation in the toxicity control reached 68.2 (day 7) to 73.3% (day 28). According to the criteria of the OECD guideline 301 D a substance can be assumed to be inhibitory, if less than 25% degradation occurred within 14 days. The test substance showed no inhibitory effects to microorganisms at a concentration of 3 mg/L.

 

In conclusion, the lowest acute aquatic toxicity value for TFMEA was the 48 h-EC50 of 19 mg/L, based on the toxicity test with the freshwater invertebrate Daphnia magna.