Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 224-698-3 | CAS number: 4454-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 3,4-dihydro-2-methoxy-2H-pyran
- EC Number:
- 224-698-3
- EC Name:
- 3,4-dihydro-2-methoxy-2H-pyran
- Cas Number:
- 4454-05-1
- Molecular formula:
- C6H10O2
- IUPAC Name:
- 2-methoxy-3,4-dihydro-2H-pyran
- Details on test material:
- - Name of test material (as cited in study report): Methoxydihydropyran
- Physical state: liquid
- Analytical purity: ca. 96%
- Impurities (identity and concentrations): 4% dimer acrolein, traces of acrolein and methylvinylether
- Expiration date of the lot/batch: not reported
- Stability under test conditions: not reported
- Storage condition of test material: not reported
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Mean weight at study initiation: 147 g for males and 133 g for females
- Fasting period before study: ca. 16 h before application
- Housing: max. 5 rats/cage
- Diet (e.g. ad libitum): Standard pellet diet for rat and mouse
- Water: ad libitum
- Acclimation period: 3-7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-23
- Humidity (%): 50-70
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- CMC (carboxymethyl cellulose)
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle (mg/ml): 100, 215, 316, 383, 464, 681, undiluted
- Amount of vehicle (if gavage): 10 ml/kg bw
DOSAGE PREPARATION: The test substance was diluted with 0.5% solution of aqueous CMC to give the desired concentrations. - Doses:
- 1000, 2150, 3160, 3830, 4640, 6810, 10000 mg/kg bw
- No. of animals per sex per dose:
- 5, except in the lowest doses where only 5 males (1000 mg/kg bw) or 5 females (2150 mg/kg bw) were used.
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Clinical signs were observed before 15 min and after 15 min, 1h, 2h, 4h, 5h and 24 h of application and daily thereafter.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs - Statistics:
- Statistical analysis were made according to Finney DJ, 1971, Probit-Analysis, p 1-150 (Published by the Syndics of the Cambridge University Press, Bentley House, 200 Euston Road, London)
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 740 mg/kg bw
- 95% CL:
- 3 530 - 3 970
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 3 890 mg/kg bw
- 95% CL:
- 3 660 - 4 140
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 3 600 mg/kg bw
- 95% CL:
- 3 280 - 3 950
- Mortality:
- 1000 mg/kg bw: 0/5 males;
2150 mg/kg bw: 0/5 females;
3160 mg/kg bw: 0/5 males and 1/5 females within 24 h;
3830 mg/kg bw: 2/5 males within 24 h and 3/5 females within 48 h;
4640 mg/kg bw: 5/5 males within 24 h and 5/5 females within 48 h;
6810 mg/kg bw: 5/5 males and 5/5 females within 24 h;
10000 mg/kg bw: 5/5 males and 5/5 females within 24 h - Clinical signs:
- other: 1000 mg/kg bw: animals showed apathy, mild to strong ataxia and closure of eyelids; from 2150 mg/kg bw onwards: animals showed apathy, reduced or no reaction to external impulses, mild to strong ataxia, narcosis like state with reduced or no upright surfa
- Gross pathology:
- In the deceased animals, reddening and hardening of intestinal mucosa were observed. In one rat of the 6810 mg/kg bw dose group, hematoma in the intestinal mucosa was observed. In the 10000 mg/kg bw dose group, dark red lung in one rat and mottled liver in 2 rats were observed. No alterations were observed in the sacrificed animals.
Applicant's summary and conclusion
- Executive summary:
This study was conducted according to guideline and is reliable without restrictions. Seven groups of male and female rats were administered with 1000, 2150, 3160, 3830, 4640, 6810, 10000 mg/kg bw of the test substance. The acute LD50 of the test substance was found to be 3740 mg/kg bw for male and female animals.
Clinical signs included apathy, reduced or no reaction to external impulses, mild to strong ataxia, narcosis like state with reduced or no upright surface reflexes, lapsed muscular tone, reduced or no reaction to pain, and decreased fecal content. These signs were reversible within 4 days after application. Normal body weight gain was observed in the surviving animals. Grossly, reddening and hardening of intestinal mucosa were observed in the deceased animals. No alterations were observed in the sacrificed animals.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.