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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed using standard test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Principles of method if other than guideline:
Objective of this study was to determine the short term toxicity or any effects of test chemical on aquatic algae Desmodesmus subspicatus and was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Remarks:
Acetone
Details on test solutions:
The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: Green algae
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available

ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed:
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Remarks on exposure duration:
±1 hour
Test temperature:
23±2°C
pH:
Test at highest concentration: 8 (change to 7.8 during tests)
Control: 8.0 (changed to 8.5 during test)
Control + acetone: 8 change to 8.2
Nominal and measured concentrations:
0, 0, 5, 10, 20, 40, 60, 80 mg/l nominal concentrations
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (K2Cr2O7)
Key result
Duration:
72 h
Dose descriptor:
other: ErC50
Effect conc.:
47.4 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95 % CI was 39.7 - 56.7 mg/l
Results with reference substance (positive control):
Results with reference substance valid
- EC50: 0.75 mg/L
Reported statistics and error estimates:
ErC50 is calculated from the inhibition of growth rate by non-linear regression using software Prism 4.0, GraphPad.
Validity criteria fulfilled:
yes
Conclusions:
The median effective concentration (ErC50) for the test substance on green algae Desmodesmus subspicatus was determined to be 47.4 mg/L.
Executive summary:

Objective of this study was to determine the short term toxicity or any effects of test chemical on aquatic algae Desmodesmus subspicatus and was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Chemical tested at the 0, 0, 5, 10, 20, 40, 60, 80 mg/l nominal concentrations. Effects on the growth rate of the organism were studied. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using non linear regression by the software Prism 4.0. The median effective concentration (ErC50) for the test substance on Desmodesmus subspicatus was determined to be 47.4 mg/L. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria. But as the chemical was readily biodegradable in water thus chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Description of key information

Objective of this study was to determine the short term toxicity or any effects of test chemical on aquatic algae Desmodesmus subspicatus and was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Chemical tested at the 0, 0, 5, 10, 20, 40, 60, 80 mg/l nominal concentrations. Effects on the growth rate of the organism were studied. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using non linear regression by the software Prism 4.0. The median effective concentration (ErC50) for the test substance on Desmodesmus subspicatus was determined to be 47.4 mg/L. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria. But as the chemical was readily biodegradable in water thus chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:
47.4 mg/L

Additional information

Objective of this study was to determine the short term toxicity or any effects of test chemical on aquatic algae Desmodesmus subspicatus and was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test) in a static system. The stock solution 100 g/l was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Chemical tested at the 0, 0, 5, 10, 20, 40, 60, 80 mg/l nominal concentrations. Effects on the growth rate of the organism were studied. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using non linear regression by the software Prism 4.0. The median effective concentration (ErC50) for the test substance on Desmodesmus subspicatus was determined to be 47.4 mg/L. This value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria. But as the chemical was readily biodegradable in water thus chemical was consider as nontoxic and not classified as per the CLP classification criteria.