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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation / corrosion
Remarks:
in vitro
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experimental Data: 19 Jan 2016 (Preliminary Data Collected 06 Jan 2016)
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study completed according to OECD 435 Guidelines, and under GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 435 (In Vitro Membrane Barrier Test Method for Skin Corrosion)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Benzene, mono-C10-13-alkyl derivatives, distillation residues, sulfonated, sodium salts
IUPAC Name:
Benzene, mono-C10-13-alkyl derivatives, distillation residues, sulfonated, sodium salts
Test material form:
liquid: viscous

In vitro test system

Test system:
other: Bovine Cornea
Source species:
other: Cow
Cell type:
other: Cornea
Cell source:
other:

Test animals

Species:
other: N/A - Macromolecular Barrier

Test system

Type of coverage:
other: In vitro
Duration of treatment / exposure:
Single Exposure

Results and discussion

In vitro

Results
Irritation / corrosion parameter:
penetration time (in minutes)
Value:
> 70.25
Vehicle controls validity:
valid
Negative controls validity:
valid
Remarks:
Citric acid
Positive controls validity:
valid
Remarks:
1.0 N Sodium hydroxide
Remarks on result:
no indication of irritation
Remarks:
Non Corrosive

Any other information on results incl. tables

The positive control, 1.0 N Sodium Hydroxide, was categorized as received and the negative control, Citric Acid, was used as a 10% solution in water. The results of both controls were considered valid.

Applicant's summary and conclusion

Interpretation of results:
other: Non-corrosive
Remarks:
Criteria used for interpretation of results: OECD GHS
Conclusions:
The material was considered non-corrosive.
Executive summary:

Benzene, mono-C10 -13 -alkyl derivatives, distillation residues, sulfonated, sodium salts was determined to be compatible with the Corrositex system. Then, a formulation of 10% test material in water 100 uL test material : 900 uL tissue culture water) was categorized by pH to determine Category 2 cut-off times should be used to determine corrosivity. 0.5 mL of the test article was added to each of four Corrositex® test vials containing biobarriers and the times required for the test article to permeate or destroy the biobarriers were recorded. A positive control (1.0 N sodium hydroxide) and a negative control (1% citric acid) were tested concurrently. The average breakthrough time for the four test material samples was >70.25 minutes. The test material was considered non-corrosive.