Registration Dossier
Registration Dossier
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EC number: 944-207-2 | CAS number: 2156592-65-1
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
An OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test) study on CASN 61789-86-4 was performed using five nominal concentrations. The calculated No Observed Adverse Effect Concentration (NOAEC) based on inhibition compared to the control was calculated to be equal to 10000 mg/L. The EC50 was > 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
- EC10 or NOEC for microorganisms:
- 10 000 mg/L
Additional information
A study has been conducted to assess the potential environmental impact and waste control for Sulfonic acids, petroleum, calcium salts (CASN 61789-86-4), in accordance to OECD Guideline 209 (Goodrich, 1994). The testing strategy incorporates the potential effect of new materials on aerobic microorganisms associated with the biological treatment of wastewater. The assessment was based on the potential of a material to inhibit the metabolism of these microbes, as indicated by the microbes consumption of oxygen. The test duration was a three-hour exposure period to the test material followed by up to ten minutes for the measurement of oxygen consumption. The study design was comprised of five nominal exposure concentrations: 1, 10, 100, 1000 and 10000 mg/L, a duplicate control group; and an assessment of the sensitivity of the inoculum used in the test to a reference toxicant (3,5- dichlorophenol). The activated sludge respiration test passed the quality control criteria for an acceptable test. The EC50 calculated for the reference toxicant was 13.0 mg/L, within the acceptable range of 5 to 30 mg/L. The two control replicates produced oxygen consumption rates within the required 15% of each other, 27 and 29 mg O2/L*h. The respiration rates of the sludge-associated microbes exposed to the five nominal concentrations of the test substance were 24.0, 24.6, 25.3, 26.7, and 28.1 mg O2/L*h. respectively. The calculated No Observed Adverse Effect Concentration (NOAEC) based on inhibition compared to the control was calculated to be equal to 10000 mg/L. The EC50 was > 10000 mg/L.
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