4-(2,6,6-trimethylcyclohex-2-ene-1-yl)-but-3-ene-2-one

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data from peer reviewed journal

Data source

Reference

Reference Type:

publication

Title:

Eye irritation study of the test chemical

Author:

Lapczynski et al

Year:

2007

Bibliographic source:

Food and Chemical Toxicology

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

To assess the ocular irritation potential of the test chemical in rabbits

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-(2,6,6-trimethylcyclohex-2-ene-1-yl)-but-3-ene-2-one

EC Number:

204-841-6

EC Name:

4-(2,6,6-trimethylcyclohex-2-ene-1-yl)-but-3-ene-2-one

Cas Number:

127-41-3

Molecular formula:

C13H20O

IUPAC Name:

4-(2,6,6-trimethylcyclohex-2-ene-1-yl)-but-3-ene-2-one

Details on test material:

- Name of test material: alpha -Ionone
- Molecular formula: C13H20O
- Molecular weight: 192.3 g/mol
- Substance type:organic
- Physical state:liquid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

no data

Test system

Vehicle:

other: No vehicle for 100% concentration and DEP for 5% concentration

Controls:

yes, concurrent no treatment

Amount / concentration applied:

A 0.1 ml aliquot of 100% and 5% the testchemical in DEP

Duration of treatment / exposure:

not specified

Observation period (in vivo):

1, 2, 4, 24, 48 and 72 h after instillation.

Number of animals or in vitro replicates:

3

Details on study design:

SCORING SYSTEM: no data

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: after instillation

Reversibility:

fully reversible within: 24 hours

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

Very slight conjunctival irritation was observed in all three rabbits at instillation, which cleared by 24 h

Any other information on results incl. tables

Table 1: Summary of the eye irritation studies

Dose	Vehicle	Result
100%	No	No irritation observed
5%	DEP	No irritation observed

 

 

Applicant's summary and conclusion

Interpretation of results:

other: not irritating

Conclusions:

Very slight conjunctival irritation was observed in all three rabbits at instillation, which cleared by 24 hours. The instillation of test chemical did not cause any ocular irritation in 3 rabbits after 72 hours of observation. The test chemical can be considered as a non irritant to rabbit eyes

Executive summary:

An eye irritation test was conducted in three rabbits to assess the ocular irritation potential of the test chemical. A 0.1 ml aliquot of 100% and 5% the test chemical in DEP was instilled into one eye of each of three rabbits with no further treatment. The untreated eye served as a control. Observations for irritation were made immediately after instillation and again at 1, 2, 4, 24, 48 and 72 h after instillation. Very slight conjunctival irritation was observed in all three rabbits at instillation, which cleared by 24 hours. The instillation of test chemical did not cause any ocular irritation in 3 rabbits after 72 hours of observation. The test chemical can be considered as a non irritant to rabbit eyes.