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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
calculation (if not (Q)SAR)
Remarks:
Migrated phrase: estimated by calculation
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from computational model developed by USEPA
Justification for type of information:
Data is from computational model developed by USEPA
Qualifier:
according to guideline
Guideline:
other: Modeling database
Principles of method if other than guideline:
ECOSAR estimates log Kow values using the US EPA's KOWWIN Program for aquatic toxicity.Toxicity of a chemical was measured on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program based upon the octanol /water partition coefficient (Kow). Data from ECOSAR class,neutral organics was used.

The estimation methods in EPI Suite™ have been developed by government, academic, and private sector researchers over many years and represent some of the best techniques currently available. The intended application domain of EPI Suite is organic chemicals, and inorganic as well as organometallic chemicals generally can be considered outside the domain.
GLP compliance:
no
Analytical monitoring:
not specified
Test organisms (species):
other: Fish
Test type:
not specified
Water media type:
not specified
Total exposure duration:
96 h
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
1.428 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Neutral organics
Conclusions:
On the basis of EPI suite, ECOSAR version 1.1, the LC 50 value for short term toxicity to fish was predicted to be 1.428 mg/l for test material in 96hrs.
Executive summary:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted.On the basis of this program, the LC 50 value for short term toxicity to fish was predicted to be 1.428 mg/l for test materialin 96 hrs.Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified as aquatic acute 2 as per the criteria mentioned in CLP regulation.

Description of key information

Short term toxicity to fish:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted.On the basis of this program, the LC 50 value for short term toxicity to fish was predicted to be 1.428mg/l for test materialin 96 hrs.Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified as aquatic acute 2 as per the criteria mentioned in CLP regulation.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
1.428 mg/L

Additional information

Short term toxicity to fish:

The toxicity of test material was evaluated for fish using a predicted study and to support the prediction data , study from secondary source of structurally similar read across substance was also used.

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to fish was predicted.On the basis of this program, the LC 50 value for short term toxicity to fish was predicted to be1.428mg/l for test materialin 96 hrs.Based on this value it can be concluded that the substance is considered to be toxic to aquatic environment and can be classified as aquatic acute 2 as per the criteria mentioned in CLP regulation.

The above prediction was further supported by data from secondary source, experimental study for test chemical was conducted according to guideline 203. Rainbow Trout (average length, 5.8 cm), were exposed to a series of 5 test concentrations of 0, 7.8, 10.9, 15.3, 21.4, or 30 mg/L dispersed in Polysorbate 80 (10 mg/L) for 96 hours at 17.1 °C. Control fish were exposed to polysorbate 80 (10 mg/L). Fish were observed twice daily for mortality and symptoms. pH values and water temperature were monitored after substance addition at 24 hour intervals. Dissolved oxygen was measured at the beginning of the experiment and at 96 hours. The LC50 value for the given test material is 10.9 mg/L.Based on the above effect concentration it can be concluded that test material is toxic to fish and can be classified as aquatic acute 2.

According to another study from secondary source , as per the guideline 203, Rainbow Trout (average length, 6-8 cm), were exposed to a series of 2 test concentrations of 0, 5, or 10 mg/L of4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-onefor 96 hours at 16+/-1°C.During experiment the LC0 was determined to be 5 mg/L while LC100 as 10 mg/L. Thus LC50 value for the given test material was considered to be between 5mg/L and 10 mg/L.Based on the above effect concentration it can be concluded that test material is toxic to fish and can be classified as aquatic acute 2.

In a data from peer reviewed handbook it was observed that,short term toxicity of test material was evaluated for Leuciscus idus fish for 96 h . The minimum leathel effect concentration (LC50) was observed to be 6.8 mg/l.

Based on the above effect concentrations it can be concluded that test material is toxic to fish and can be classified as aquatic acute 2.