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Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Remarks:

Chemical Oxygen Demand

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

Principles of method if other than guideline:

Guideline followed

GLP compliance:

yes

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

2 h

Details on study design:

None

Reference substance:

other: Potassium hydrogen phthalate solution (Merck-Nr. 11769)

Remarks on result:

not measured/tested

Key result

Parameter:

COD

Value:

862 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate was determined to be 199 mg O2/l.

None

Validity criteria fulfilled:

yes

Interpretation of results:

other: Chemical Oxygend Demand determined

Conclusions:

The COD of the test substance FAT 20042/D was determined to be 862 mg O2/g.

Executive summary:

FAT 20042/D was investigated for Chemical Oxygen Demand in a study carried out according to DEV DIN 38'409 - H 41 -1 (1980) corresponding to 92/96/EEC C.6. The test substance was tested with a sample weight of 5.80 + 5.74 mg. The test substance FAT 20042/D in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. Based on the findings, the COD of FAT 20042/Dwas 862 mg O2/g, whereas the COD of the reference substance was 199 mg O2/I.

Reference 2

Endpoint:

biodegradation in water: screening test, other

Remarks:

Biochemical Oxygen Demand determined

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

Deviations:

not specified

Principles of method if other than guideline:

Guideline followed

GLP compliance:

no

Specific details on test material used for the study:

None

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

None

Duration of test (contact time):

5 d

Details on study design:

None

Reference substance:

not specified

Preliminary study:

None

Test performance:

None

Remarks on result:

not measured/tested

Details on results:

None

Key result

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Results with reference substance:

None

None

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The BOD5 of FAT 20042/D was determined to be 0.0 mg O2/g.

Executive summary:

The biochemical oxygen demand (BOD5) of FAT 20042/D is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20°C. Based on the findings of the study, the BOD5 of FAT 20042/D :was determined to be 0 mg O2/g.

Taking the value of the corresponding COD test into account, the calculated BOD5/COD- ratio is less than 0.5 which indicates that the substance is not subject to biodegradation.

Description of key information

The test substance Acid Red 195 was considered to be not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The biodegradation potential of Acid Red 195 was investigated in studies that determined its Biochemical Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD).

FAT 20042/D was investigated for Chemical Oxygen Demand in a study carried out according to DEV DIN 38'409 - H 41 -1 (1980) corresponding to 92/96/EEC C.6. The test substance was tested with a sample weight of 5.80 + 5.74 mg. The test substance FAT 20042/D in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. Based on the findings, the COD of FAT 20042/D was 862 mg O2/g, whereas the COD of the reference substance was 199 mg O2/I.

The biochemical oxygen demand of FAT 20041/D is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C. Based on the results, the BOD of FAT 20042/D : 0 mg 02/g.

Based on these results the corresponding BOD5/COD quotient for FAT 20042/D is equivalent to zero, so no biodegradation is expected.

 

Other supporting studies for FAT 20042/B (1979) and FAT 20042/A (1979) were also performed with following results -

COD - 508.17 mg O2/g, 1038 mg O2/g

BOD - 0 mg O2/g, 24 mg O2/g.

In all the cases, the BOD5/COD ratio for Acid Red 195 was <0.5, hence the substance is considered to be not readily biodegradable.