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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
26.-28.11.1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
Part I, 1984
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
No data.
Vehicle:
no
Details on test solutions:
No data.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain/clone: n.a.
- Source: daphnids were bred in the laboratories of the TF Product Ecology under standardized conditions
- Age of parental stock (mean and range, SD): < 24 h
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
136 mg/L CaCO3
Alkalinity: 0.8 mmol/L
Test temperature:
21 °C
pH:
7.8 - 8.2
Dissolved oxygen:
Test media were saturated with oxygen at the beginning of the test.
Salinity:
no data
Conductivity:
no data
Nominal and measured concentrations:
nominal: 0.1, 0.18, 0.32, 0.58 and 1.0 ppm
measured mean concentration: 0.08, 0.14, 0.25, 0.45, 0.78 ppm
Details on test conditions:
TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 250 ml beakers containing 100 ml test medium
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): n.a.
- Renewal rate of test solution (frequency/flow rate): /
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: bidistilled water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Intervals of water quality measurement: at beginning and at end of the test

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility after 24 and 48 h

VEHICLE CONTROL PERFORMED: yes (blank control)

RANGE-FINDING STUDY
- Test concentrations: no details reported
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.17 other: ppm
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% confidence limit
Remarks:
0.14 - 0.19
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.08 other: ppm
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
0.45 other: ppm
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Test evaluations were done according to the guidelines by visual assessment of the mobility of the daphnids after 24 and 48 hours.
The EC50 was calculated by using the Probit model and by graphical determination on logarithmic probability paper.
The concentration of the test substance at the beginning and at the end of the test was analysed by the analytical Service Klybeck.
Results with reference substance (positive control):
EC50 (24 h) = 0.9 ppm (range: 0.8 - 1.5 ppm)

1) Analytical results of test substance measurements:

Nominal concentration [ppm]

Concentration start [ppm]

Concentration end [ppm]

Mean value* [ppm]

0.10

0.08

0.07

0.08

0.18

0.14

0.13

0.14

0.32

0.26

0.23

0.25

0.58

0.47

0.42

0.45

1.00

0.80

0.75

0.78

* used for calculation of EC50 value

2) Oxygen concentration and pH

The oxygen concentration and the pH was controlled at the beginning and at the end of the test.

Start:        - Oxygen: saturated

- pH: 8.0 (highest test concentration)

- Temp.: 21° C

End:

Nominal Conc. [ppm]

0.1

0.18

0.32

0.58

1.0

Blanc

Oxygen [%]

96

96

96

97

96

97

pH

7.9

7.8

7.9

7.9

7.9

7.7

Temp. [°C]

21

21

21

21

21

21

 

3) Influence of the Test Item on the immobilisation of Daphnia magna:

Nominal concentration [ppm]

No. of animals

No. of responses

Immobility [%]*

24 h

48 h

24 h

48 h

0 (Blank)

10

0

0

0

0

10

0

0

0.08

10

0

0

0

0

10

0

0

0.14

10

1

4

5

20

10

0

0

0.25

10

9

9

95

95

10

10

10

0.45

10

9

10

90

100

10

9

10

0.78

10

10

10

100

100

10

10

10

 * calculated for 20 animals

Validity criteria fulfilled:
yes
Conclusions:
The 48-h EC50 of the test item to Daphnia magna under the conditions of the OECD test guideline 202 is 0.17 ppm.
Executive summary:

The 48h acute toxicity of the test item to Daphnia magna was studied under static conditions. Daphnids were exposed to 0 (blank control), 0.08, 0.14, 0.25, 0.45, 0.78 ppm (measured concentration). Mortality/immobilisation were observed daily.  The 48h EC50was 0.17 ppm. The 48h EC0 based on mortality/immobilisation was 0.08 ppm. 

 

Based on the results of this study, the test item would be classified in category Chronic 1 in accordance with the classification system of the GHS.

 

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

 

Results Synopsis

Test Organism Age (e.g. 1stinstar): < 24 h

Test Type: Static

 

EC0:  0.08 ppm

EC50:  0.17 ppm                       95% C.I.:  0.14 -0.19 ppm

Endpoint(s) Effected:  mortality/immobilisation

Description of key information

The 48h acute toxicity of the test item to Daphnia magna was studied under static conditions in accordance with OECD test guideline 202. Daphnids were exposed to 0 (blank control) and mean measured concentrations of 0.08, 0.14, 0.25, 0.45, 0.78 ppm. The 48h EC50 was 0.17 ppm. 

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.17 mg/L

Additional information