1-Ethoxy-2,3-difluoro-4-(trans-4-pentylcyclohexyl)-benzene

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Apr 12, 2004 - Jul 14, 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study was performed according to GLP and the methods applied are fully compliant with OECD TG 301C.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-Ethoxy-2,3-difluoro-4-(trans-4-pentylcyclohexyl)-benzene

EC Number:

603-009-3

Cas Number:

124729-02-8

Molecular formula:

C₁₉H₂₈F₂O

IUPAC Name:

1-Ethoxy-2,3-difluoro-4-(trans-4-pentylcyclohexyl)-benzene

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):activated sludge, micro organisms from a domestic waste water treatment plant- OriginMunicipal sewage treatment plant-Conditioning:The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and centrifuged again. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

According to guideline

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

The biodegradation in % was calculated based on the ThOD of the test item.

Test performance:

The reference item (Aniline) was sufficiently degraded to 57%, 68%, 71%, and 72 % after an incubation for 7, 14, 21 and 28 days respectively.The validity criterion that the degradation should be > 60 % after 14d was fulfilled.

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (test mat. analysis)

Value:

-1

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.