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EC number: 269-522-6 | CAS number: 68259-31-4
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Key study: Givaudan (1996); Ready biodegradability (OECD 301 B in sealed vessels): Result = Not readily biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
In the key study, the ready and ultimate biodegradability of the test material was investigated under GLP conditions and in accordance with the standardised guideline OECD 301 B using closed vessels. The study was assigned a reliability score of 1 in accordance with the criteria detailed by Klimisch et al. (1997).
The ready and ultimate biodegradability of the test material was determined by measuring CO₂ evolution in the headspace of each test vessel over 28 days using secondary effluent from an un-acclimatised activated sludge plant. The initial concentration of test material was 10 mg/L organic carbon (nominal). Samples were taken and the evolution of CO₂ measured on Days 4, 8, 14, 22, 24 and 28. Control vessels were run concurrently.
The test material achieved -7.4 % degradation over the 28 day study period. It therefore failed the test and therefore cannot be classed as being readily or ultimately biodegradable.
Supporting information is available in the form of a study conducted to determine the inherent biodegradability of the test material using a sealed vessel test under GLP conditions. The study was assigned a reliability score of 2 in accordance with the criteria detailed by Klimisch et al. (1997).
The sealed vessel test is a CO₂ production test based on OECD Guideline 301B and studies conducted by the OECD aimed at harmonising the various tests of ready biodegradability. The ready test guidelines specify a maximum test duration of 28 days. If the test duration is extended to 56 days then the test is no longer an assessment of ready and ultimate biodegradability but is indicative of inherent and ultimate biodegradability, albeit under modified ready test conditions. This version of the sealed vessel test is not a recognised OECD/EEC test of inherent biodegradability. However, the test system is closed and it is therefore suitable for the examination of volatile materials.
The inherent and ultimate biodegradability of the test material was determined by measuring CO₂ evolution in the headspace of each test vessel over 56 days using secondary effluent from an un-acclimatised activated sludge plant. The initial concentration of test material was 10 mg/L organic carbon (nominal). Samples were taken and the evolution of CO₂ measured on Day 56. Control vessels were run concurrently.
The test material achieved -4.4 % degradation over the 56 day study period. It therefore failed the test and therefore cannot be classed as being inherently or ultimately biodegradable.
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