Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 September 2008- 18 Septemebr 2008.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Test performed in accordance with OECD guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Qualifier:
according to
Guideline:
ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
Qualifier:
according to
Guideline:
other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
Substance type: white powder
- Physical state: solid
- Analytical purity: 99.80%
- Expiration date of the lot/batch: 31 Jan 2009
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
<= 28 mg/L
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks:
at 28 mg/l ( NOEC)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC 50 for both growth rate reduction ( ERC50: 0 -72h) and yield inhibition ( EYC50: 0 -72h) was beyond 28 mg/l.
Executive summary:

Under the conditions of the study with pseudokirchneriella subcapitata, the test substance did not reduce growth rate of this freshwater algae species at 28mg/l (NOEC). Yield inhibition was statically inhibited by 22% ( NOEC < 28 mg/l).

The EC 50 for both growth rate reduction ( ERC50: 0 -72h) and yield inhibition ( EYC50: 0 -72h) was beyond 28 mg/l.