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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 15, 1995 to August 16, 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted 4 April 1984
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
29th Dec. 1992
Deviations:
no
Principles of method if other than guideline:
Guidelines followed
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Code number: FAT 20063/C
Batch No.: 3.46
Purity: 81.9 %
Appearance: solid
Solubility: < 10 g/l [in water]
Storage: room temperature
Expiration date: 04/1999
Analytical monitoring:
yes
Details on sampling:
All test medium samples for the analytical determinations were taken from the approximate center of the test vessels (after mixing the medium) and kept at -18 °C to -25 °C until analysis.
Vehicle:
yes
Remarks:
Tween 80 (Polyoxyethylensorbitan-monooleat) in a concentration of 5 ppm
Details on test solutions:
Stock solution
0.5 g test substance were mixed with 200 ml test medium in a beaker, was homogenized for 5 minutes by ultrasonic treatment and filled up to 500 ml with test medium.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: Product Ecology Division of Ciba-Geigy Ltd.,Basel

Breeding :
- Cultures of Daphnia magna are held in glass vessels containing approx. 3.0 litre of reconstituted water at 18 - 22°C. The water is renewed partially three times per week. The Daphnia are fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a TETRAMIN-extract in such quantités that the food will be consumed
within 24 hours.

Pretreatment :
- 24 hours before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 1000 µm sieve.

For each test concentration 20 Daphnia divided in two groups of 10 animals were used.

Experimental conditions
48 hours at nominal 18-22°C (during the test max. ± 1°C), 12 hours light /12 hours dark. The Daphnia were exposed to the test substance in 250 ml beakers containing 80-100 ml test medium with 10 animals per beaker. All tests were run in duplicate.
Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h
Hardness:
231 mg/l CaCO3
Test temperature:
Start: 21 °C, End: 21.6 - 22.0 °C
pH:
Start: 5.8 (in the highest test concentration), End: 6.8 - 7.8
Dissolved oxygen:
Start: 99 %, End: 95 - 96 %
Nominal and measured concentrations:
45, 100 and 220 mg test article/l (nominal concentrations);
Reference substance (positive control):
yes
Remarks:
potassium dichromate (periodically checks to evaluate the functionality of the test systems have been performed only)
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
173 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95 % confidence limit after 48 hours: 145-201 mg/l (Spearman-Kaerber's Method).
Details on results:
In all test concentrations no alteration of the test substance was observed after 48 hours. During the test the actual test concentrations in the analysed test media were in the range of 115-118% of the nominal values. Therefore, the test substance was sufficiently stable in the test medium and the reported results are related to the nominal test concentrations. No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations up to 100 mg/l. Thus, the NOEC and EC0 value after 48 hours were determined to be 100 mg/l. The EC100 value could not be determined, but was clearly higher than 220 mg/l.

The EC50 value after 48 hours was calculated as 173 mg/l.
Results with reference substance (positive control):
Reference substance
The quality of the Daphnia was checked by determining the EC50 value (24 h) for potassium dichromate (March 29, 1995); EC50: 0.96 mg/l.

None

Validity criteria fulfilled:
yes
Conclusions:
The EC50 value after 48 hours was calculated as 173 mg/l.
Executive summary:

The acute toxicity of FAT 20063/C to Daphnia magna was determined in a 48 hours static test according to OECD Guideline 202 and EU Method C.2. The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland. Due to the results of a pretest the nominal concentrations for the main test were selected as 45, 100 and 220 mg test article/l, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1.0 g/l stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours. During the test, the actual test concentrations in the analysed test media were in the range of 115-118% of the nominal values. Therefore, the test substance was sufficiently stable in the test medium and the reported results are related to the nominal test concentrations. No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations up to 100 mg/l. Thus, the NOEC and EC0 value after 48 hours were determined to be 100 mg/l. The EC100 could not be determined, but was clearly higher than 220 mg/l. The EC50 value after 48 hours was calculated as 173 mg/l.

Description of key information

The EC50 value after 48 hours was calculated as 173 mg/l.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
173 mg/L

Additional information

The acute toxicity of FAT 20063/C to Daphnia magna was determined in a 48 hours static test according to the OECD Guideline 202 and EU Method C.2. The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland. Due to the results of a pretest the nominal concentrations for the main test were selected as 45, 100 and 220 mg test article/l, a vehicle and a blank control without any additions. The test concentrations were prepared from a 1.0 g/l stock solution. In all test concentrations no alteration of the test substance was observed after 48 hours. During the test, the actual test concentrations in the analysed test media were in the range of 115-118 % of the nominal values. Therefore, the test substance was sufficiently stable in the test medium and the reported results are related to the nominal test concentrations. No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations up to 100 mg/l. Thus, the NOEC and EC0 value after 48 hours were determined to be 100 mg/l. The EC100 could not be determined, but was clearly higher than 220 mg/l. The EC50 value after 48 hours was calculated as 173 mg/l.