Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
October 18, 1994 to January 06, 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Version / remarks:
1992
Deviations:
no
Principles of method if other than guideline:
Guideline followed
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
Identification: FAT 20063/D TE
Lot: BS-BOP 01-15
Appearance: Dark blue powder
Specific details on test material used for the study:
- Code number: FAT 20063/C
- Batch-Nr.: 3.46
- Purity: 81.9 %
- Appearance: solid
- Solubility: >10 g/I [in water]
- Storage: room temperature
- Expiration date: 04/99

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Activated sludge of a communal sewage treatment plant Ara Reinach collected on 18.10.94
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
150 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Reference substance
Reference substance:
diethylene glycol
Remarks:
CIBA-Nr. FAT 90019/E .Fluka No. 32170

Results and discussion

% Degradation
Key result
Parameter:
% degradation (DOC removal)
Value:
0
Sampling time:
28 d
Remarks on result:
other:
Details on results:
Adsorption:
The adsorption of the test substance FAT 20063/C after 3 hours was: 1 %

Biodegradation:
The biodegradation of the test substance FAT 20063/C after 28 days was: 0 %

Total Elimination:
(regarding the adsorption and the biodegradation) The total elimination of the test substance FAT 20063/C over the whole test period of 28 days was (average of 2 tests running in parallel): 1 %

BOD5 / COD results

Results with reference substance:
The biodegradation, corrected by the blank control and measured as DOC (mg/l) is: 98 % (7th day & average of 2 tests running in parallel)

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The test substance did not show any biodegradation under the test conditions.
Executive summary:

The inherent biodegradability of FAT 20063/C was determined in a modified Zahn-Wellens Test according to OECD Guideline 302B, in compliance with GLP. The test substance FAT 20063/C and the reference substance Diethylenglycol, respectively, were tested in concentrations of 150 mg/l DOC. The adsorption of the test substance after 3 hours was estimated to be 1 %. The biodegradation of the test substance FAT 20063/C after 28 days was 0 %. Hence, the total elimination of the test substance over the whole test period of 28 days was 1 %. The biodegradation of the reference substance after 7 days was estimated to be 98 %. Based on the findings of the study, it can be concluded that the test substance did not show any biodegradation under the test conditions.