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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
genetic toxicity in vivo
Remarks:
Type of genotoxicity: other: meiotic effects
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented study which meets basic scientific principles. BPA is a constituent of the reaction mass so that its hazard data are relevant for the assessment of the reaction mass.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2008

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Experiment 1: Oocytes from untreated MF1 mice (n=4-12 per group) were matured in vitro with 0, 50, 100, 200, 400, or 800 ng/ml, 4 or 10 µg/ml BPA (0.22-43.8 µM) and evaluated for meiotic progression.
Experiment 2: C57Bl x CBA/Ca F1 hybrid female mouse pups at 22 days of age (n=4 per group, usually) were administered by gavage 0, 20, 40, or 100 ng/g BPA on 7 consecutive days. Oocytes were isolated and matured ex vivo, then evaluated for meiotic progression.
GLP compliance:
no
Type of assay:
other: assessment of meiotic progression

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse
Strain:
other: MF1; C57Bl x CBA/Ca hybrid
Sex:
female

Administration / exposure

Route of administration:
other: in vitro; oral gavage
Vehicle:
corn oil

Results and discussion

Additional information on results:
Experiment 1: At the highest BPA dose (10 ug/ml), meiotic progression, nuclear maturation, and chromosomal constitution were increased. This dose also affected spindle formation, distribution of pericentriolar material, and chromosome alignment on the spindle, causing significant meiotic arrest. BPA did not increase hyperploidy at meiosis II at any tested concentration.
Experiment 2: BPA exposure was not associated with any significant effects on meiotic progression, spindle abnormalities, or aberrant chromosome behaviour.

Any other information on results incl. tables

Experiment 1: BPA did not increase hyperploidy at meiosis II at any tested concentration.

Experiment 2: BPA exposure was not associated with any significant effects on meiotic progression, spindle abnormalities, or aberrant chromosome behaviour.

Applicant's summary and conclusion

Conclusions:
Experiment 1: BPA did not increase hyperploidy at meiosis II at any tested concentration.

Experiment 2: BPA exposure was not associated with any significant effects on meiotic progression, spindle abnormalities, or aberrant chromosome behaviour.
Executive summary:

Experiment 1: Oocytes from untreated MF1 mice (n=4-12 per group) were matured in vitro with 0, 50, 100, 200, 400, or 800 ng/ml, 4 or 10 ug/ml BPA (0.22-43.8 µM) and evaluated for meiotic progression. At the highest BPA dose (10 µg/ml), meiotic progression, nuclear maturation, and chromosomal constitution were increased. This dose also affected spindle formation, distribution of pericentriolar material, and chromosome alignment on the spindle, causing significant meiotic arrest. BPA did not increase hyperploidy at meiosis II at any tested concentration.

Experiment 2: C57Bl x CBA/Ca F1 hybrid female mouse pups at 22 days of age (n=4 per group, usually) were administered by gavage 0, 20, 40, or 100 ng/g BPA on 7 consecutive days. Oocytes were isolated and matured ex vivo, then evaluated for meiotic progression. BPA exposure was not associated with any significant effects on meiotic progression, spindle abnormalities, or aberrant chromosome behaviour. The authors concluded that low chronic BPA exposure in vivo does not appear to pose a risk for induction of errors in chromosome segregation at first meiosis in mouse oocytes.