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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Description of key information

In accordance with OECD 209 (2010) and according to GLP principles the influence of the substance on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The substance was not toxic to waste water (activated sludge) bacteria at a concentration of 1000 mg/L (NOEC). This corresponds to a NOEC of 390 mg/L expressed as solid content. The 3h-EC50 was determined to be approximately 2600 mg/L, which corresponds to a 3h-EC50 of approximately 1000 mg/L expressed as solid content. In addition, in several ready biodegradability studies it was found that the substance is not toxic to the inoculum (up to 94.2 mg/L).

Key value for chemical safety assessment

EC50 for microorganisms:
2 600 mg/L
EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information

In accordance with OECD 209 (2010) the influence of the substance on the respiration rate of activated sludge was investigated after a contact time of 3 hours. Two experiments were performed. In experiment 1, a combined limit/range-finding test, concentrations of 10, 100 and 1000 mg/l, corresponding to 3.9, 39 and 390 mg solid/l, were tested. The highest concentration was tested in triplicate, lower concentrations consisted of one replicate. In addition, blank-controls and a nitrification control of the sludge were included. Furthermore, an abiotic control and the highest concentration with a nitrification inhibitor were tested. In experiment 2, a limit test, a concentration of 2600 mg/l, corresponding to 1000 mg solid/l was tested. The concentration was tested in triplicate. In addition, a blank-control (2 replicates) was included. The combined limit/range-finding test showed no inhibition of the respiration rate at all tested concentrations. There was no significant oxygen uptake from abiotic processes and the results at 1000 mg/l with a nitrification inhibitor showed a very slight heterotrophic inhibition of the respiration rate. The limit test showed 48% inhibition of the respiration rate. Thus, based on the first experiment the NOEC was determined to be 1000 mg/l, corresponding to 390 mg solid/l and based on the second experiment the EC50 was estimated to be approximately 2600 mg/l, corresponding to 1000 mg solid/l. The batches of activated sludge were checked for sensitivity in both experiments by testing the reference substance 3,5-dichlorophenol, which showed normal sensitivity.