2,2'-(1,4-phenylene)bis[4-[(4-methoxyphenyl)methylene]oxazol-5(4H)-one]

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: early study short report, but giving the main details of design and performance, intensified conditions with still not irritant result

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

extented exposure, add. scarified skin

GLP compliance:

no

Remarks:

pre dates GLP regulation

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-(1,4-phenylene)bis[4-[(4-methoxyphenyl)methylene]oxazol-5(4H)-one]

EC Number:

257-055-0

EC Name:

2,2'-(1,4-phenylene)bis[4-[(4-methoxyphenyl)methylene]oxazol-5(4H)-one]

Cas Number:

51202-86-9

Molecular formula:

C28H20N2O6

IUPAC Name:

2,2'-(1,4-phenylene)bis[4-(4-methoxybenzylidene)-1,3-oxazol-5(4H)-one]

Test animals

Species:

rabbit

Strain:

Himalayan

Details on test animals or test system and environmental conditions:

TEST ANIMALS
SPF-Albino-Himalayan-Kaninchen
Strain: Hoe:HIMK(SPFWiga)

- Weight at study initiation: 1,5-2 kg
- Housing: single
- Diet (e.g. ): ad libitum
- Water (e.g. ad libitum): ad libitum

Test system

Type of coverage:

occlusive

Preparation of test site:

clipped

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

500 mg/ site

Duration of treatment / exposure:

24 h

Observation period:

72 h

Number of animals:

6

Details on study design:

TEST SITE
- Area of exposure: 6,25 cm²
- Type of wrap if used: surgical gauze + occlusive tape

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no
- Time after start of exposure: 24 h

SCORING SYSTEM:
§ 1500.41 Federal Register 38, No. 187, 27. 9. 1973, p 27019

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 72 h

Score:

0

Max. score:

0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The test item is considered not irritant.

Executive summary:

The fur of 6 rabbits (weight; 1,5 to 2,0 kg) was clipped free of hair with aa electrical clipper at two adjacent sites (each 3 x 3 em)

One of the two sites was sacrified additionally.

Pieces fot surgigal gauze (2,5 x 2.5 cm²) loaded with 500 mg of the powder were fixed using adhesive tape to the application sites. Sites were covered occlusively with 6 -8 cm wide PVC foiles fixed to the trunk with an elastic bandage.

Exposure lasted for 24 hours. Immediately afterwards observations were made and repeated 48 and 72 hours after the start of exposure.

Based on the findings the irritation index was calculated according to § 1500.41 Federal Register 38, No. 187, 27. 9. 1973, p 27019, Result: Der test item was tolerates by all animals without any effect (Index = 0) and is therefore considered not irritant.