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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Based on the pass levels (60 % of bio-degradation in the 10-day window period), the test item cannot be considered as readily biodegradable, as only 8.69 % degradation was achieved during the test.

Key value for chemical safety assessment

Additional information

The ready biodegradability of FAT 40866/A TE was tested using the CO2 Evolution Test. The test item was added to two test vessels at the concentration of 40 mg/L of mineral medium (equivalent to 15.1 mg of Total Organic Carbon/L). Two control treatments containing only the inoculum, one positive control treatment and one toxicity control treatment containing the test item and the positive control were also tested.

All the treatments were added with inoculum from secondary effluent treatment plant receiving predominantly domestic sewage.   Treatment mixtures were aerated for 29 days with carbon dioxide (CO2) free air. The CO2 released was trapped in a series of bottles containing barium hydroxide, which were connected to the outlet of each test vessel. The residual barium hydroxide was measured on the 3, 5, 7, 9, 11, 16, 21, 25 and 29thday after the initiation of the test.   There was 8.69 % degradation of test item during the treatment period while, the percent degradation of reference item was 97.91 % and the toxicity control was 65.55 % at the end of the test.   The test fulfilled all the validity criteria.  

Based on the pass levels (60 % of bio-degradation in the 10-day window period), the test item cannot be considered as readily biodegradable, as only 8.69 % degradation was achieved during the test.