Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: The test follows the EU Directive 79/831/EEC, Annex V, part C, which is comparable to the OECD guideline 202.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): p- Fluoro-w-chloroacetophenone
- Physical state: Solid
- Analytical purity: > 99%
- Lot/batch No.: 23473/86/2

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
A defined amount of the substance was weighed out and suspended directly in the test medium by stirring (24 h at 20 °C) . The nominal concentration of the stock solution was 100 mg/l.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source:Daphnia magna STRAUS, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation: 2 - 24 hours old

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.70 ± 0.5 mmol/L
Test temperature:
19.6 - 20.8 °C
pH:
7.9 - 8.3
Dissolved oxygen:
8.1 - 8.8 mg/L
Nominal and measured concentrations:
Nominal test concentrations: control, 0.02, 0.04, 0.08, 0.16, 0.32, 0.63, 1.25, 2.5, 5 mg/L
Recovery rate (study initiation, unstocked): -, 120, n.m., n.m., n.m., n.m., 91, n.m., n.m., n.m., 88 %
Recovery rate (study end, unstocked): -, 60, n.m., n.m., n.m., n.m., 85, n.m., n.m., n.m., 99 %
Recovery rate (study end, stocked): -, -, n.m., n.m., n.m., n.m., 60, n.m., n.m., n.m., 80 %
(n.m. = not measured)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 ml glass tubes with flat bottom, 10 ml test volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline;
M4 medium with the properties:
Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.8 - 1.0 mmol/L
Ratio Ca/Mg ions: about 4 : 1
pH value: 7.5 - 8.5
Conductivity: 600 - 700 microS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, diffuse light (about 2-7 microeinstein/m*m*s (400-750 nm))

EFFECT PARAMETERS MEASURED :
Observations: mobile daphnids after 24 and 48 h
Measurements: pH and oxygen content after 0 and 48 h; temperature: 0, 24, 48 h.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 0.3 - 0.5 CL
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
1.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion